Acrylamide/Bis-acrylamide 19:1, MP Biomedicals™

Acrylamide/Bis-acrylamide 19:1, Molecular Biology Grade, 4X Crystallized, Solution

Brand:  MP Biomedicals 11BIAC1902

Product Code. 11426970

Description

• AMV Reverse Transcriptase is an RNA-dependent DNA polymerase.
• The enzyme is composed of two polypeptides, α and β.
• The molecular weight of the α subunit is 68,000 Da and that of the β subunit 92,000 Da.
• It is widely used for first and second strand synthesis of complementary DNA (cDNA) from mRNA templates for cloning or for hybridization probes.
• Such synthesis requires a primer, commonly oligo(dT) bound to the 3'-poly(A) region of mRNA.
• Polymerization proceeds in the 5' to 3' direction with no 5' to 3' exonuclease activity.
• Under the right conditions, high yields of full length cDNA can be obtained using AMV reverse transcriptase.
• AMV Reverse Transcriptase is suitable for synthesis of cDNA using specific primers, at temperatures of 55 to 60°C.
• This high reaction temperature permits transcription of RNA containing secondary structure.
• Free of contaminating endonucleases, exonucleases, and ribonuclease.

Specifications

• Chemical Name or Material: Bis-Acrylamide Gel
• Physical Form: Liquid
• Quantity: 1 L
• Concentration or Composition (by Analyte or Components): 40% (w/v)