Invitrogen™ ACK1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA596151

Product Code. 16558991

Description

Immunogen sequence: KANFSTNNSN PGARPPPPRA TARLPQRGCP GDGPEAGRPA DKIQMAMVHG VTTEECQAAL QCHGWSVQRA AQYLKVEQLF GLGLRPRGEC HKVLEMFDWN LEQAGCHLLG SWGPAHHKR Positive Samples: SW620, HT-29, MCF7, Raji; Cellular Location: Cell junction, Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endosome, Membrane, Nucleus, Peripheral membrane protein, adherens junction, clathrin-coated pit, clathrin-coated vesicle.

TNK2 (ACK) (activated Cdc42-associated kinase) is a non-receptor tyrosine kinase that signals downstream of Cdc42. TNK2 is known to be involved in tumorigenesis and metastasis, particularly in prostate cancer. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

Specifications

• Antigen: ACK1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TNK2
• Gene Alias: ACK; ACK1; Ack-1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; Cdc42 GTPase-inhibiting protein; Cdgip; Non-receptor protein tyrosine kinase Ack; p21cdc42Hs; P21cdc42Hs kinase; Pyk1; Tnk2; tnk2 {ECO:0000250; tyrosine kinase non receptor 2; tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2; UniProtKB:Q07912}
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10188, 303882, 51789
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.21 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: O54967, Q07912, Q5U2X5
• Gene Symbols: TNK2
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 920-1038 of human TNK2 (NP_0057723).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG