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  6. Thermo Scientific™ PepMap™ 300 C18 HPLC Columns

Thermo Scientific™ PepMap™ 300 C18 HPLC Columns

Product Code. 11312213 Shop All Thermo Scientific Products
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Length (Metric):
5 mm
150 mm
Diameter (Metric):
0.075 mm
0.3 mm
1 mm
Column Format:
Analytical Column
Analytical Column, NanoViper
Trap Cartridge
Unit Size:
Each
Pack of 5
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Length (Metric) Diameter (Metric) Column Format unitSize
11312213 150 mm 0.075 mm Analytical Column Each
13481208 150 mm 1 mm Analytical Column, NanoViper Each
13471208 150 mm 0.3 mm Analytical Column, NanoViper Each
11342223 5 mm 0.3 mm Trap Cartridge Pack of 5
17224444 150 mm 0.075 mm Analytical Column, NanoViper Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
5 options
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Product Code. 11312213 Supplier Thermo Scientific™ Supplier No. 163574

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Description

Use this nanocolumn, packed for LC-MS/MS peptide mapping, protein identification, biomarker discovery, and systems biology applications.

This is the standard column for high-resolution, high-sensitivity protein identification for proteomics and biomarker discovery and verification. The Thermo Scientific™ Acclaim™ PepMap™ 300 C18 LC Columns is ideal for:

  • High-resolution peptide mapping of tryptic, natural, and synthetic peptides
  • LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology

High loading capacity

 

Acclaim PepMap columns are exceptionally suitable for the analysis of low abundant peptides often found in complex proteomics samples.

The Acclaim PepMap C18 300Å column 5μm Particle Size is available in 150mm lengths in 75, 300, 1000, and classic 75μm I.D. to allow for tuning throughput, sensitivity, and amount of sample available.

 

  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

Columns are available with Thermo Scientific™ Dionex™ nanoViper Fingertight Fittings:

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar
  • About Nano, capillary, and micro Acclaim PepMap columns

Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.

TRUSTED_SUSTAINABILITY

Specifications

Column Format Analytical Column
Diameter (Metric) 0.075 mm
Flow Rate 200 mL/min
Length (Metric) 150 mm
Max. Pressure 7250 psi (500 bar)
Particle Size 5 μm
pH 2 to 8
Pore Size 300 Å
Surface Area 100 m²/g
Temperature 60°C
USP Type L1
Stationary Phase C18
Product Line nanoLC
Packing Material Spherical, Fully Porous, Ultrapure Silica
Carbon Load 9%
Column Type Reversed Phase
Endcapped Yes
Phase Reversed Phase
Type Analytical Column
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Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

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