Invitrogen™ Human AAK1, GST Tag Recombinant Protein

Product Code. 17168785

Brand: Invitrogen™ A33370

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This AAK1 recombinant human protein (1-465) was expressed in insect cells. The purified catalytic domain (1-510) of AAK1 can be used in a number of different applications, but has been specifically validated for use with our LanthaScreen™ Kinase binding assay technology to identify novel kinase inhibitors. AAK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. Validated for use with the LanthaScreen™ Eu Kinase Binding assay Available in multiple pack sizes for convenient use Also available in our SelectScreen Kinase Profiling Service Protein Form: Recombinant, Catalytic.

AAK1 serine/threonine kinase is a member of the NAK family and plays a regulatory role in endocytosis. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.

Specifications

• Accession Number: Q2M2I8
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 25% glycerol, 0.25mM DTT, 0.1mM PMSF, 150mM NaCl, 0.1mM EDTA, 10mM glutathione and no preservative; pH 7.5
• Gene ID (Entrez): 22848
• Molecular Weight (g/mol): 77.3 kDa
• Name: Human AAK1, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 5530400K14Rik; 9630042K20; Aak1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; AU067724; AU067726; BC028270; C79663; D6Ertd245e; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570; mKIAA1048; R75501; RGD1563580
• Common Name: AAK1
• Gene Symbol: AAK1
• KinaseFamily: NAK Kinase Family
• Protein Length: 1-510
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-510
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic
• Form: Liquid