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Eurofins DiscoverX A-Raf Protein, Active

Product Code. 17320854
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Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
This item is not returnable. View return policy

Product Code. 17320854

Brand: Eurofins DiscoverX 14956

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This item is not returnable. View return policy

Description

Active, N-terminal GST-tagged, recombinant, human A-Raf, for use in Kinase Assays

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

GenBank U01337.1 The recombinant protein contains the amino acid substitution Y301D and Y302D with reference to GenBank U01337.1. These mutations provide constitutive activation of the enzyme.

Specifications

Formulation mg/mL of enzyme in 40 mM Tris/HCl pH 8.0, 200 mM NaCl, 0.08 mM EGTA, 0.024% Brij-35, 4 mM glutathione, 20% (v/v) glycerol, 0.8 mM benzamidine, 0.16 mM PMSF, 0.08% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 65 kDa
Quantity 10 μg
Species Human
Recombinant Recombinant
Protein Tag N-terminal GST
Expression System Recombinant enzyme expressed in Sf21 insect cells
Protein Form Truncated
Purity or Quality Grade ≥47%
Protein A-Raf, active
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