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Invitrogen™ 6x-His Tag Monoclonal Antibody (AD1.1.10), FITC
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Product Code. 11560073
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Quantity:
100 μg
Unit Size:
100µg
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11560073 100 μg 100µg
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Product Code. 11560073 Supplier Invitrogen™ Supplier No. MA181891

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331.36 GBP valid until 2026-04-10
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Description

Mouse Monoclonal Antibody

MA1-81891 recognizes proteins containing the motiff H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts aginst all His-tagged proteins tested so far. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
TRUSTED_SUSTAINABILITY

Specifications

Antigen 6x-His Tag
Applications Flow Cytometry, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone AD1.1.10
Concentration 0.1 mg/mL
Conjugate FITC
Formulation PBS with 1% BSA and 0.9% sodium azide; pH 7.4
Gene Alias 6His tag; 6X His; His Tag; Histidine Tag
Host Species Mouse
Immunogen PAX6 transcription factor linked to histidine tag.
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Tag
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