R&D Systems™ Recombinant Human Common gamma Chain Fc Avi Protein

Product Code. 18705626

Brand: R&D Systems™ AVI11045050

Description

Biotinylated IL-2 R gamma Fc Chimera Avi-tag

Measured by its binding ability in a functional ELISA. When Recombinant Human CD25/IL-2R alpha Protein (Catalog # 223-2A ) and Recombinant Human IL-2R beta Protein (Catalog # 223-2B ) are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL-2 R gamma Fc Chimera Avi-tag (Catalog # AVI11045) that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL-2 Protein (Catalog # 202-IL ).

Specifications

• Accession Number: P31785.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3561
• Molecular Weight (g/mol): MolecularWeight-observed: 85-95 kDa, under reducing conditions.; MolecularWeight-theroretical: 56 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Common gamma Chain/IL-2 R gamma protein Human IL-2 RG (Leu23-Asn254) (N-terminus) Accession # P31785.1 IEGRMD Human IgG1 Fc (Pro100-Lys330) Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD132, CD132 antigen, CIDX, combined immunodeficiency, X-linked, common cytokine receptor gamma chain, common gamma-chain, cytokine receptor common subunit gamma, gamma(c), IL-2 R gamma, IL-2 receptor subunit gamma, IL2R gamma, IL-2 R subunit gamma, IL2RG, IL-2 RG, IMD4, interleukin 2 receptor, gamma, Interleukin-2 receptor subunit gamma, p64, SCIDX, SCID x 1, severe combined immunodeficiency
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Common gamma Chain/IL-2 R gamma