R&D Systems™ Recombinant Human IL-15 Protein

Product Code. 18799336

Brand: R&D Systems™ 247ILB025/CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554). Specific activity is for reference purposes only and is not routinely tested.

Specifications

• Accession Number: P40933.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS. Reconstitute at 100μg/mL in sterile PBS.
• Gene ID (Entrez): 3600
• Molecular Weight (g/mol): 10 kDa, under reducing conditions.
• Quantity: 25 μg
• Source: E. coli-derived human IL-15 protein Asn49-Ser162
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 °C to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: IL15, IL-15MGC9721, interleukin 15, interleukin-15
• Common Name: IL-15
• Gene Symbol: IL15
• Biological Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.3-2.6ng/mL. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Asn49-Ser162
• Purity or Quality Grade: >97%, by SDS-PAGE with silver staining.