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  5. Invitrogen™ 20-Hydroxyecdysone ELISA Kit

Invitrogen™ 20-Hydroxyecdysone ELISA Kit

Product Code. 17184853
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
Pack of 10
Pack of 96
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Product Code. 17184853

Brand: Invitrogen™ EIAHYD

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Description

ELISA

The 20-Hydroxyecdysone Competitive ELISA quantitates 20-Hydroxyecdysone (20E) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The 20-Hydroxyecdysone Competitive ELISA research-use-only kit is designed to quantitatively measure 20-Hydroxyecdysone independent of species. A 20-Hydroxyecdysone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit 20-Hydroxyecdysone antibodies and 20-Hydroxyecdysone-peroxidase conjugate is added to the wells. The 20-Hydroxyecdysone-peroxidase conjugate and any 20-Hydroxyecdysone in the sample will compete to bind to the anti-rabbit 20-Hydroxyecdysone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 20-Hydroxyecdysone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 20-Hydroxyecdysone present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

20-Hydroxyecdysone is the most widely occurring ecdysteroid in both plant and animal species. It controls cell death during metamorphosis of Drosophila melanogaster.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 20 E; C27H44O7; ecdysterone
Assay Range 102.4 to 25,000 pg/mL
Assay Sensitivity 197 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Fecal Extract, Plasma, Serum, Supernatant, Urine
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 9%
Intraassay CV 10
Kit Contents Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
Storage Requirements -20°C
Target 20-Hydroxyecdysone
Test Time 45 mins
Total Assay Time 2 hrs 30 mins
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