Invitrogen™ 14-3-3 beta Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA529689

Product Code. 13493917

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, YWHAB shRNA-transfected 293T. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (89%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.

Specifications

• Antigen: 14-3-3 beta
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: YWHAB
• Gene Alias: 1300003C17Rik; 14-3-3 alpha; 14-3-3 protein beta; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha, N-terminally processed; 14-3-3 protein beta-subtype; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; brain protein 14-3-3, beta isoform; epididymis secretory protein Li 1; GW128; HEL-S-1; HS1; hypothetical protein GW128; KCIP 1; KCIP1; KCIP-1; Prepronerve growth factor RNH-1; protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; QccE-16215; RP1-148E22.1; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; unnamed protein product; YWHAA; Ywhab
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54401, 7529
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P31946, Q9CQV8
• Gene Symbols: YWHAB
• Immunogen: Recombinant protein encompassing a sequence within the center region of human 14-3-3 beta. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG