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Invitrogen™ 123count eBeads™ Counting Beads

Product Code. 15526296
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100 tests
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100 tests
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Contains Sodium azide
Product Code. 15526296 Supplier Invitrogen™ Supplier No. 01123442

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This item is not returnable. View return policy
Contains Sodium azide

Description

Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.

  • Supplied at a known concentration in 0.05% Tween-20 with 2 mM sodium azide
  • 10mL aqueous suspension
  • Collecting carefully suspended and measured quantities of cell:bead mixtures on a flow cytometer makes the ratiometric enumeration of cells possible
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Equipment) Flow Cytometer
No. of Tests 100 tests
Quantity 100 tests
Product Line eBeads
Product Type Cell Counting Bead

Frequently Asked Questions (FAQs)

I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do?

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

I want to count my cells using flow cytometry. How do I do this?

Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration.
What does yield and purity mean in reference to the MagniSort kits? How are each of these parameters calculated?

Yield is the percentage of target cells harvested based on the number of cells theoretically available in the starting population. To calculate yield, stain a sample of unsorted and sorted cells with antibodies to identify the target cells, and count the total number of target cells using 123count eBeads (Cat. No. 01-1234-42) on the cytometer. Yield = # of sorted target cells, # of target cells unsorted.

Purity is the percentage of cells harvested that are target cells. Purity can be directly generated by flow cytometric analysis of sorted samples. To obtain purity, stain the sorted cells with antibodies to identify the target cells and analyze by flow cytometry. Gate on live, single cells, and subsequently gate on the target cell population. Purity is the percentage of target cells in the live, single-cell gate.

For Research Use Only. Not for use in diagnostic procedures.

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