R&D Systems™ Recombinant Escherichia T7 phage RNA polymerase Protein, CF

Product Code. 30306770

Brand: R&D Systems™ 11662T7100

Description

His-tag

Measured by its ability to produce 4.1 kB RNA per agarose gel electrophoresis from a defined linearized DNA plasmid template quantified using a fluorescent detection assay. The RNA yield is >2500 ng, as measured under the described conditions.

Specifications

• Accession Number: P00573.2
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Sodium Phosphate, NaCl, TCEP and Glycerol.
• Gene ID (Entrez): 1261050
• Molecular Weight (g/mol): MolecularWeight-observed: 89-98 kDa, under reducing conditions, MolecularWeight-theroretical: 100 kDa
• Quantity: 100 μg
• Source: E. coli-derived e. coli T7 phage RNA polymerase protein Asn2-Ala883 with a N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: T7p07
• Conjugate: Unconjugated
• Protein: T7 phage RNA polymerase