R&D Systems™ Rat IL-18 BPa (NP_445826) VersaClone cDNA

Product Code. 18739908

Brand: R&D Systems™ RDC2553

Description

This shuttle vector contains the complete ORF for the gene of interest, along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector.

• Il18bp interleukin 18 binding protein [ Rattus norvegicus (Norway rat) ]
• The IL-18 BPa/IL18BP insert is sequence verified and the entire plasmid DNA and insert translated sequences are provided.
• Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity.
• Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18.
• The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.
• IL-18 BPa functions as an inhibitor of the proinflammatory cytokine, IL18.
• It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced Thelper type 1 immune responses.
• It is constitutively expressed and secreted in mononuclear cells.
• Elevated level of IL-18 BPa is detected in the intestinal tissues of patients with Crohn's disease.
• For reasearch use only. Not for use in humans.

Specifications

• Gene: rIl18bp
• Accession Number: NP_445826
• Concentration: 10 μg at 0.2 μg/μL
• Formulation: cDNA is provided in 10 mM Tris-Cl, pH 8.5
• Insert Type: 601 bp
• Gene ID (Entrez): 10068
• Content And Storage: Store the unopened product at -20°C to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Species: Rat
• Quantity: 10 μg