Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Brand: Thermo Scientific™

Manufacturer Part Number: 88882

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg

Product Code. 12126510

Quantity Price
1 £ 556.0 / 40 reactions
EU Stock 14
Add to basket

Description and Specification


Formulation Six-component kit
Isotopic Labeling No
Kit Contents Accessory Proteins, 100uL
Source Material DNA
Sufficient For 40 reactions of 25uL each
Description Pierce 1-Step Human Coupled IVT Kit - DNA
Quantity 40 rxn kit

The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100μg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.


Functional – uses the human translational machinery to express active proteins

  • Convenient – perform transcription and translation in a single step
  • High performance – greater yields compared to rabbit reticulocyte in vitro translation
  • Reliable – express proteins that fail in rabbit reticulocyte systems

Better Than Traditional Methods:

  • HeLa cell-free extracts are capable of expressing proteins with post-translational modifications
  • Accurate translation delivers full-length protein compatible with downstream applications
  • Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements


Complete kits contain HeLa cell lysate, accessory proteins, reaction mix, nuclease-free water, expression vector and a GFP-positive control vector


30°C incubator or water bath

Recommended for:

Express proteins to measure enzyme activity; Perform mutational analysis; Express protein for use in gel mobility shift assays; Perform co-immunoprecipitation; Express cytotoxic proteins; Perform unnatural amino acid labeling