Miscellaneous Molecular Reagents

Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution

Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Thermo Scientific™ DTT (dithiothreitol)

Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.

Thermo Scientific™ Pierce™ Negative Ion Calibration Solution

Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.

Thermo Scientific™ Pierce™ LTQ ESI Positive Ion Calibration Solution

Calibrate LTQ, LTQ Orbitrap, LXQ and LCQ FLEET mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Thermo Scientific™ IPTG, dioxane-free

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Thermo Scientific™ Pierce™ Trypsin Protease, MS Grade

Ensure maximum activity and stability in proteomic applications with this chemically modified, highly purified porcine trypsin.

Thermo Scientific™ X-Gal Solution, ready-to-use

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Invitrogen™ IPTG

Inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.

Thermo Scientific™ TriTrack DNA Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X TriTrack DNA Loading Dye.

Thermo Scientific™ Glycerol

Perform a variety of protein and molecular biology applications. Thermo Scientific™ Pierce Glycerol is highly purified.

Thermo Scientific™ RNA Gel Loading Dye (2X)

Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.

Thermo Scientific™ Pierce™ HeLa Protein Digest Standard

Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Thermo Scientific™ Amino Acid Standard H

Calibrate HPLC analysis of protein hydrolysates using this calibration standard mixture of 18 amino acids, each at 2.5 micromoles/mL in 0.1N HCl.

Thermo Scientific™ Pierce™ BSA Protein Digest, MS grade

Calibrate and maintain quality control for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples using the carefully validated Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade.

Thermo Scientific™ Orange DNA Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels withThermo Scientific™ 6X Orange Loading Dye.

Thermo Scientific™ DMEM for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ Triple Quadrupole Calibration Solution

Calibrate Triple Stage Quadrupole (TSQ) mass spectrometers using a mixture of high purity, ionizable components specifically designed for positive mode calibration.

Thermo Scientific™ MassRuler DNA Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X MassRuler DNA Loading Dye.

Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Macherey-Nagel™ TLC Spray Visualization Reagents

Used for postchromatographic detection reactions in thin-layer chromatography. Macherey-Nagel™ TLC Spray Visualization Reagents are a selection of frequently used spray reagents that are suited for spraying or dipping TLC plates. Glass laboratory sprayer (mfr. no. 814101) sold separately.

Thermo Scientific™ Ethidium Bromide Solution (0.625 mg/mL)

Visualize DNA fluorescently in a variety of molecular biology applications using these dilute (0.625mg/mL) or concentrated (10mg/mL) ethidium bromide solutions.

Thermo Scientific™ IPTG Solution, ready-to-use

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg

Study protein expression by quantitative mass spectrometry using amine-labeling compounds of equal total mass (isobaric) but different reporter ions.

Thermo Scientific™ EDTA (0.5 M), pH 8.0

Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.

Thermo Scientific™ TMT10plex™ Isobaric Label Reagent Set

For multiplex quantitative protein expression analysis by mass spectrometry

Thermo Scientific™ Pierce™ 6 Protein Digest, equimolar, LC-MS grade

Calibrate and maintain quality control for mass spectrometry (MS) analysis of proteomic samples using the Thermo Scientific™ Pierce™ 6 Protein Digest Standard, Equimolar, LC-MS Grade, a highly validated equimolar protein digest mixture.

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay

Measure total peptide concentration by a colorimetic microplate method that provides improved sensitivity, reproducibility and ease using the Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay.

Thermo Scientific™ Yeast DNA Extraction Kit

Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.

Thermo Scientific™ Hydrochloric Acid Solution, Sequencing Grade

Constant-boiling 6N HCl solution, supplied in 1mL ampules, used to acid-hydrolyze proteins (protein hydrolysis) for HPLC amino acid composition analysis.

  spinner