Biological Buffers

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

4LT NUCLEASE-FREE WATER 4X1 LITER

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free 1 LT TE, PH 8.0 1L STORE AT ROOM TEMPERATURE

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 2.5 - 5Store in Fridge at 4C

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 1 LT 0.5 M EDTA, PH 8.0 1L STORE AT ROOM TEMPERATUre

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels NOVEX TRIS-GLYCINE SDS RUNNING BUFFER (10X DENATURing) . store at room temperature

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. 100mL Blocker Casein in TBS

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Use with Novex Tris-Glycine Gels TRISGLY SDS SAM BUF (2X)20ML

Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats X10 BOLT SAMPLE RED AGT

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)

Invitrogen™ Novex™ NativePAGE™ Cathode Buffer Additive (20X)

For running NativePAGE Gels NATIVEPAGE 20X CATHODE BUF ADDNativePAGE Cathode Buffer Additive (20X) is added

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents

Commonly used buffering agent 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ UltraPure™ RNase A (20mg/mL)

Isolates RNA-free DNA 10 ML RNASE A 10ML SHELF LIFE = 3650 PACKINGinstructions = RRR Storage = Fridge 4C

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 1000 MLsolution, 1000 ml

Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)

Use with Novex Tricine Gels TRICINE SDS RUN BUF10X 500MLNovex Tricine SDS Running Buffer (10X)

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

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