Biological Buffers

Invitrogen™ Ambion™ SSC (20X)

Ready-to-use, certified RNase-free 500 ML 20X SSC 500ML STORE AT ROOM TEMPERATURE

Alfa Aesar™ MES, 0.5M buffer soln., pH 7.0

Chemical Name or Material: MES buffer soln. CAS: 145224-94-8 MDL Number: MFCD00283848 100ML MES, 0.5M buffer soln., pH 7.0 100ml

Invitrogen™ UltraPure™ Dithiothreitol

High and low concentration applications DITHIOTHREITOL (DTT)

Invitrogen™ Elongase™ Enzyme Mix

Amplifies both long and short DNA templates 100 RXN ELONGASE ENZYME MIX 100 REACTIONS 00Store at -20C

Invitrogen™ Ambion™ Nuclease-Free Water (not DEPC-Treated)

Autoclaved, membrane-filtered 500 ML NUCLEASE-FREE WATER (NOT DEPC-TREATED) (1x 500mL) 500mL Store at room temperature

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 500 ML WESTERN BLOT STRIPPING REAGENT, RESTORE500mL

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 5 LT SUPERBLOCK(R) BLOCKING BUFFER IN PBS 5LStore in Fridge at 4C

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

Chemical Name or Material: HEPES CAS: 7365-45-9 Purity Grade: Molecular Biology Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Powder/Solid Assay: ≥99% Assay Percent Range: ≥99 % Molecular Formula: C8H18N2O4S Formula Weight: 238.3 Synonym: N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid 1KG HEPES (Fine White Crystals) for Molecular Biology,

Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions. 500 ML GENTLE AG/AB ELUTION BUFFER, IMMUNOPURE(DMSO) 500mL Store in fridge at 4C

PIPES, disodium salt, 99%, ACROS Organics™

Chemical Name or Material: PIPES, disodium salt Assay: 98.5 to 101.5% (dry basis, acidimetry) Assay Percent Range: 99% Molecular Formula: C8H16N2Na2O6S2 Formula Weight: 346.33 Synonym: 1, 4-Piperazinediethanesulfonic acid disodium salt 250GR PIPES, disodium salt, 99%

Invitrogen™ Ambion™ Gel Loading Buffer II (Denaturing PAGE)

Compatible with denaturing polyacrylamide gels 10 ML GEL LOADING BUFFER II (DENATURING PAGE)10mL Store at -20 C

Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT SAMPLE REDUCING AGENT

Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Built-in hot start 5000 RXN PCR SUPERMIX 21 HIGH FIDELITY PLATINUM(R)5000 reactions Store at -20C

Invitrogen™ Novex™ ZOOM™ Focusing Buffer pH 3-7

For use with ZOOM IPG Strips FOCUSING BUFFER PH 3-7, LIQUID ZOOM(R) 10ML STOREat 2° to 8°C

Alfa Aesar™ MOPSO, 0.2M buffer soln., pH 6.5

Chemical Name or Material: MOPSO buffer soln. CAS: 79803-73-9 MDL Number: MFCD00070007 100ML MOPSO, 0.2M buffer soln., pH 6.5 100ml

Thermo Scientific™ Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent. GUARDIAN HRP CONJ STABILIZER 200ML

Invitrogen™ Novex™ IEF Sample Buffer pH 3-7 (2X)

Optimized pre-mixed IEF buffers reduce variability and ensure consistent results IEF SAM BUF PH 3-7 (2X) 25MLNovex® IEF Sample Buffer pH 3-7 (2X) is optimized

Alfa Aesar™ TES, 0.2M buffer soln., pH 7.0

Chemical Name or Material: TES buffer soln. CAS: 70331-82-7 MDL Number: MFCD00065482 250ML TES, 0.2M buffer soln., pH 7.0 250ml

Invitrogen™ Ambion™ tYeast RNA (10mg/mL)

Co-precipitant to aid in nucleic acid recovery 500 UL YEAST TRNA (10 MG/ML) 500µL STORE AT -20 C

Invitrogen™ Ambion™ THE RNA Storage Solution

Features to minutesimize base hydrolysis of RNA THE RNA STORAGE SOLN. 10 X 1MLTHE Ambion® RNA Storage Solution is a buffer that

Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer (2X)

Ideal for native running conditions with NuPAGE Tris-Acetate gels TRISGLY NATIVE SAM BUF 20ML

Alfa Aesar™ MES, 0.5M buffer soln., pH 8.0

Chemical Name or Material: MES buffer soln. CAS: 145224-94-8 MDL Number: MFCD00283848 250ML MES, 0.5M buffer soln., pH 8.0 250ml

Invitrogen™ Ambion™ Water-Saturated Phenol, pH 6.6

Ready-to-use, safe alternative to preparing crystalline phenol solutions 100 ML WATER SATURATED PHENOL, PH 6.6 (RAISE TOpH 7.9 with includedbuffer) 100mL Store at 4°C

Alfa Aesar™ Kinase buffer III (5X)

For cellular and molecular biology applications 100ML Kinase buffer III (5X)

Alfa Aesar™ TES, 0.2M buffer soln., pH 7.5

Chemical Name or Material: TES buffer soln. CAS: 70331-82-7 MDL Number: MFCD00065482 250ML TES, 0.2M buffer soln., pH 7.5 250ml

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

Invitrogen™ Novex™ Bolt™ Antioxidant

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML BOLT ANTIOXIDANT

Alfa Aesar™ BICINE, 0.5M buffer soln., pH 9.0

Chemical Name or Material: BICINE buffer soln. CAS: 150-25-4 MDL Number: MFCD00004295 250ML BICINE, 0.5M buffer soln., pH 9.0 250ml

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