Biological Buffers

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Storage Requirements: Store at room temperature. 4LT NUCLEASE-FREE WATER 4X1 LITER

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels NOVEX TRIS-GLYCINE SDS RUNNING BUFFER (10X DENATURing) . store at room temperature

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility 15 MG GLYCOBLUE 15MG STORE AT -20°C

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Use with Novex Tris-Glycine Gels TRISGLY SDS SAM BUF (2X)20ML

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 1 LT 0.5 M EDTA, PH 8.0 1L STORE AT ROOM TEMPERATUre

Invitrogen™ DEPC-Treated Water

Storage Requirements: Store at room temperature. DEPC-TREATED WATER 5 X 100 MLAmbion® DEPC-treated water is certified

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 4.5 TO 5.425mL Store in Fridge at 4C

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. 100mL Blocker Casein in PBS

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

Chemical Name or Material: HEPES CAS: 7365-45-9 Purity Grade: Molecular Biology Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Powder/Solid Assay: ≥99% Assay Percent Range: ≥99 % Molecular Formula: C8H18N2O4S Formula Weight: 238.3 Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid 5KG HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents

Chemical Name or Material: HEPES Buffer Name Note: 1M Solution, pH 7.3 CAS: 7365-45-9 CAS: 7732-18-5 Purity Grade: Molecular Biology Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Liquid Molecular Formula: C8H18N2O4S Formula Weight: 238.197 Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology

Invitrogen™ MgCl2 (1M)

Ready-to-use, certified RNase-free 1 M MAGNESIUM CHLORIDE 100MLMgCl2solution, 100 ml

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. X5 TBS blocking buffer, Dry Blend, SuperBlock 00

  spinner