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Reverse Transcriptases

Individual reverse transcriptases and associated reaction buffers suitable for reverse trancription PCR (RT-PCR) reactions and processes.

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Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
2
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Invitrogen™ MultiScribe™ Reverse Transcriptase

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

3
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Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.

Concentration 200 U/μL
4

Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 30 to 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
5

Thermo Scientific™ Maxima H Minus Reverse Transcriptase Promotion

A highly thermostable, high processivity reverse transcriptase lacking RNase H activity

Content And Storage -20°C
Concentration 5X
For Use With (Application) cDNA Synthesis,RT-PCR,Gene Expression