Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.

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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Pricing and Availability
Specifications

Flow Rate	4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type	Pre-packed Column
Content And Storage	4°C to 30°C
Description	HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
Support Type	6% Highly Cross-linked Agarose

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Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Pricing and Availability
Specifications

Buffer	Lysis Buffer
Content And Storage	Upon receipt store at 4°C
Inhibitors	Protease Inhibitor
Form	Liquid
Product Type	Protease Inhibitor Cocktail
Reagent Type	Protease Inhibitor
Product Line	Halt

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Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

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Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 M_r in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Pricing and Availability

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Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Assay	End-point Chromogenic Assay
Type	Endotoxin Quantitation Kit
Target	Endotoxins
Content And Storage	• Lyophilized amebocyte lysate • E.coli endotoxin standard • Chromogenic substrate • Endotoxin-free water Store at 2–8°C upon receipt.
Format	Kit
Assay Sensitivity	0.01 EU/mL
Assay Range	0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line	Pierce
Detection Method	Colorimetric

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Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation

Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.

Pricing and Availability
Specifications

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein G covalently bound to Dynabeads
Surface Functionality	Protein G
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein G
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads

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Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

Pricing and Availability
Specifications

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein A covalently bound to Dynabeads
Surface Functionality	Protein A
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein A
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads

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Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Pricing and Availability
Specifications

Particle Size	1 μm
Ligand Type	Streptavidin
Type	Magnetic Affinity Bead
Target	Biotin
Content And Storage	Upon receipt store at 4°C. Product is shipped on an ice pack.
Protein Form	Recombinant
Form	Liquid
Concentration	Slurry: 10 mg/mL, 1% solids
Product Line	Pierce

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Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Pricing and Availability
Specifications

Buffer	Lysis Buffer
Content And Storage	Upon receipt store at 4°C
Inhibitors	Protease Inhibitor
Form	Liquid
Product Type	Protease Inhibitor Cocktail
Reagent Type	Protease Inhibitor
Product Line	Halt

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Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Pricing and Availability
Specifications

Content And Storage	Store in a cool, dry, well-ventilated area, protected from direct sunlight.
Enzyme	Nuclease
Form	Liquid
Product Type	Cell Lysis Enzyme
Reagent Type	Enzyme for Cell Lysis
Product Line	Pierce

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Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Pricing and Availability
Specifications

Buffer	Lysis Buffer
Content And Storage	Upon receipt store at 4°C
Inhibitors	Protease Inhibitor, Phosphatase Inhibitor
Form	Liquid
Product Type	Protease and Phosphatase Inhibitor Cocktail
Reagent Type	Protease Inhibitor, Phosphatase Inhibitor
Product Line	Halt

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Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.

Pricing and Availability
Specifications

Content And Storage	Store at room temperature.
Column Type	Empty Column, Polyethylene Filter
Product Line	Pierce

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Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

Pricing and Availability
Specifications

Particle Size	1 μm
Ligand Type	Protein A/G
Type	Magnetic Affinity Bead
Target	Antibody
Content And Storage	Upon receipt store at 4°C. Product shipped with an ice pack.
Protein Form	Recombinant
Form	Liquid
Product Line	Pierce

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Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Pricing and Availability
Specifications

Content And Storage	Store at Room Temperature
Form	Powder
Product Type	Detergent
Reagent Type	Detergent (Pure)

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Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown

Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets.

Pricing and Availability
Specifications

Content And Storage	Dynabeads His-Tag Isolation and Pulldown beads are supplied in 20% ethanol. Store at 2°C to 8°C.
Final Product Type	His-Tagged Protein
Color	Brown
Binding Property	1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads
Material	Magnetic Polystyrene
Concentration	40 mg/mL
For Use With (Application)	Isolation of His-tagged Recombinant Proteins
High-throughput Compatibility	Yes
Regulatory Status	For Research Use Only
Ligand Type	Nitrilotriacetic acid (NTA)
Type	Magnetic Beads
Target	His-tagged proteins
Format	Magnetic beads
For Use With (Equipment)	KingFisher Sample Purification System, DynaMag magnets
Sample Type	Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts
Diameter (Metric)	1 μm
Certifications/Compliance	ISO9001 and ISO13485
Shelf Life	36 months from date of manufacture
Product Line	Dynabeads

Protein Purification

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