Protein Analysis Reagents
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Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
| Assay | BCA Assay |
|---|---|
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Product Type | Protein Quantitation Assay |
| For Use With (Application) | Solution-based Detection, Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
| MWCO | 7.0 kDa |
|---|---|
| Column Type | Size-exclusion, Proprietary Resin |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Zeba |
Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays.
| Product Line | Qubit |
|---|---|
| Detection Method | Fluorescence |
Thermo Scientific™ RIPA Lysis and Extraction Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
| Content And Storage | Upon receipt store at 4°C. |
|---|---|
| Format | Liquid |
| Product Type | Extraction Buffer |
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor |
| Form | Liquid |
| Product Type | Protease Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein G covalently bound to Dynabeads |
| Surface Functionality | Protein G |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein G |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Thermo Scientific™ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Grade | MS |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Invitrogen™ Streptavidin, horseradish peroxidase conjugate
The streptavidin, horseradish peroxidase (HRP) conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic HRP substrates.
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 488 |
| Emission | 517 nm |
| Excitation | 494 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein A covalently bound to Dynabeads |
| Surface Functionality | Protein A |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein A |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Thermo Scientific™ Pierce™ HeLa Protein Digest Standard
Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
| Form | Solid |
|---|---|
| Final Product Type | Peptides |
| Product Type | Protein Digest Standard |
| For Use With (Equipment) | Mass Spectrometer |
| Reagent Type | Standard |
| Starting Material | Peptides |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
| Label Type | Biotin & Analogs |
|---|