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Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Restriction Enzymes (725)
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1 – 15 of 573 results
1

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

EDGE
2

Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Cut Site CTCTTC(1/4)^
Content And Storage Store at -20°C.
Form Liquid
Enzyme Eam1104I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity 3000 U, 150 μL
Type IIS RE Yes
Incubator Temperature 37°C
Concentration 20 U/μL
For Use With (Application) In vitro Transcription
Inactivation Temperature 65°C
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Research Category Drug Development
Compatible Buffer 10X Tango Buffer
Optimal Reaction Temperature 37°C
Product Line TheraPure™
3

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

4
Promotions Available

Invitrogen™ RNase Cocktail™ Enzyme Mix

Endonuclease-free, exonuclease-free, protease-free

EDGE
Shipping Condition Dry Ice
Product Type RNAse Cocktail Enzyme Mix
Enzyme RNase
Product Line Ambion™
5

Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix

Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

Shipping Condition Dry Ice
Content And Storage Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Product Type LR Clonase Enzyme Mix
Enzyme LR Clonase
Compatible Buffer Enzyme Buffer
Product Line Clonase™, Gateway™
6

Thermo Scientific™ AarI (2 U/μL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme AarI
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer Unique Buffer (10X Buffer AarI)
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 2 U/μL
7

Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

EDGE
Polymerase DNA Polymerase I
Product Type Klenow Fragment
Compatible Buffer Restriction Enzyme, PCR, RT Buffer
Concentration 5 U/μL
8
Promotions Available

Thermo Scientific™ DraI (10 U/μL)

Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

EDGE
9

Thermo Scientific™ NheI (10 U/μL)

The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).

EDGE
Concentration 10 U/μL
10

Thermo Scientific™ PscI (PciI) (10 U/μL)

The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI).

EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme PscI (PciI)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer Tango
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
11

Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).

EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Eco81I (Bsu36I)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer Tango
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
12

Thermo Scientific™ HinfI (10 U/μL)

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Hinf I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer R
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
13

Thermo Scientific™ PfeI (TfiI) (10 U/μL)

Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).

EDGE
14

Thermo Scientific™ Hin6I (HinP1I) (10 U/μL)

Cut at G^CGC sites with Hin6I (HinP1I) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI).

EDGE
15

Thermo Scientific™ PagI (BspHI) (10 U/μL)

The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).

EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme PagI (BspHI)
Methylation Sensitivity Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer O
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL