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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 13 of 13 results
1

Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane

Nitrocellulose membrane, with a 0.45 μm pore size, is suitable for proteins of a wide range of molecular weights.

EDGE
2

Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

3

Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent

Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane

Product Type ECL Western Blotting Detection Reagent
Storage Requirements 18°C to 30°C
For Use With (Application) Routine and confirmatory Western blotting
4

Thermo Scientific™ Miser™ Antibody Extender Solution NC

Enable Western blot detection with much less primary antibody by treating nitrocellulose membrane with this reagent.

5

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Content And Storage Upon receipt store solutions at 4°C Products are shipped at ambient temperature.
Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric
6

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Product Type Transfer Buffer
7

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

8

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-mouse

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

9

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

10

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-goat

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

11

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-rabbit

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

12

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

13

Invitrogen™ Novex™ Reversible Membrane Protein Stain Kit

Used for determining protein transfer efficiency after blotting