Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Gibco™ DPBS, no calcium, no magnesium
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DPBS, no calcium, no magnesium
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | DPBS |
Gibco™ DPBS, calcium, magnesium
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS, calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
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Dilution | 1 X |
Content And Storage | 2° to 8°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 7 to 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Gibco™ DPBS (10X), calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS (10X), calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Capacity | 500 mL |
pH | 4.3 to 4.7 |
Shelf Life | 24 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2700 to 3200 mOsm/kg |
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS (10X), no calcium, no magnesium
Dilution | 10 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 6 to 7 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2500 to 2900 mOsm/kg |
Gibco™ HEPES (1M)
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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HEPES (1M)
Thermo Scientific™ Dry-blend Buffered Packs
Our quality control ensures that every pack of dry-blend buffers yields consistent solutions without weighing or pH adjustment. Simply empty contents of one foil envelope pack into a beaker, add appropriate solvents, and stir to dissolve.
Form | Powder |
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Product Line | BupH™ |
Gibco™ PBS, pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS, pH 7.2
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 280 to 320 mOsm/kg |
Gibco™ ACK Lysing Buffer
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Thermo Scientific Chemicals Krebs-Ringer Solution, HEPES-buffered
Standard physiological buffer that is suitable for various calcium imaging experiments. It is also used as a preserving agent.
Solubility Information | Not miscible or difficult to mix in water. |
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Packaging | Plastic bottle |
Physical Form | Liquid |
Chemical Name or Material | Krebs-Ringer Solution |
TSCA | Yes |
Recommended Storage | Ambient temperatures |
Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, ultrapure, 99.9%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00004679 |
SMILES | C(C(CO)(CO)N)O |
Synonym | TRIS |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS (10X), pH 7.4
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
Gibco™ HEPES, 1M Buffer Solution
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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HEPES, 1M Buffer Solution
Absorbance | UV light |
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Packaging | 1 Each |
Physical Form | Liquid |
pH | 7.2 to 7.5 |
Certificate of Analysis | FDA, ISO 13485 standards |
Chemical Name or Material | HEPES buffer |
Concentration | 1 M |
For Use With (Application) | Mammalian cell culture |
Cell Culture Tris-Buffered Saline (10X), Corning™
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
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Supports traceability with guaranteed access to certificates and proactive change notifications.
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With low endotoxin content
Gibco™ Opti-Plex™ Complexation Buffer
Opti-Plex Complexation Buffer is a cGMP-manufactured, chemically defined, animal origin-free (AOF), methionine-free, biotin-free, low-protein transfection complexation buffer based on Opti-MEM I Reduced Serum Medium