Eppendorf

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Eppendorf is a leading life science company that develops instruments, consumables and services for handling liquids, samples and cells in laboratories worldwide – with the commitment to deliver the best solution for managing your most precious research. Because, after 75 years, the mission of the company is just as valid and relevant as it ever was: “Eppendorf contributes to improving human living conditions.” So, since 1945, and for tomorrow as well, join the Eppendorf family and choose products that are a perfect fit for all your day-to-day laboratory work.

About Eppendorf

The Eppendorf product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers and DNA amplification equipment, as well as ultra-low temperature freezers, fermenters, bioreactors, CO2 incubators, shakers and cell manipulation systems. Consumables such as pipette tips, test tubes, microtitre plates and single-use bioreactor vessels complement the range of highest-quality premium products.


Eppendorf products are used widely in academic and commercial research laboratories, especially within the pharmaceutical, biotechnological, chemical and food sectors. The Eppendorf range is also perfect for clinical, forensic and environmental laboratories, and also the wider industrial environment wherever process analysis, production, and quality assurance protocols are being performed.

Accuracy, reliability, experience, innovation - characteristics that customers all over the world associate with Eppendorf.


Eppendorf's broad product range covers three competence areas:

Liquid Handling

Liquid handling is a core process in practically every life science laboratory. Naturally, this is one of Eppendorf's core areas of expertise. It's hard to imagine a laboratory without our completely reliable devices and consumables.

Cell Handling

From manipulation to cultivation - in microbiology and cell culture - more and more scientists from research to production have been using our cell handling products.

Sample Handling

As a scientist, your samples are your most valuable assets. Eppendorf knows this. Ensuring their safe handling and developing innovative solutions for a wide variety of sample processing requirements is what drives us.


Videos

Eppendorf 75 Years

We don’t know what will come. But we know that you will find out. Together we can look towards a future that inspires and improves people´s living conditions. 

From Harvesting to Cell Manipulation

Are you familiar with the varied selection of Eppendorf products for applications in the modern cell biology laboratory?  


ThermoMixer-Take Advantage

No matter your lab or applications, the Eppendorf ThermoMixer family integrates into your workflow with ease. Improve your assay results by mixing and incubating your samples at the same time – with highest vessel flexibility.

Get to know our customers

Transforming science for tomorrow: Watch how the University of East London keeps their position as one of the most modern universities of the UK - with Eppendorf at their side. 


Molecular Biology – Life in Process

Are you familiar with the varied selection of Eppendorf products for applications in the modern molecular biology laboratory? 

Precise and Repeatable Pipetting of Problem Liquids

Pipetting seems easy, but some tips and tricks can help you to get consistent and reproducible results - even with difficult liquids.


Reproducible DNA Amplification in PCR

Nowadays, PCR can be robust and easy—but you can still run into difficulties. Make sure to get the most out of your PCR with some simple tricks.

Aerosols in the Lab

Aerosols are colloidal systems of droplets and / or solid particles suspended in air. Aerosols can be found almost everywhere in the world and we inhale them continuously, so we should differentiate between harmful and harmless aerosols. The most important aerosols in the laboratory environment are bio-aerosols.


Resources

Literature Resources


White Papers


Posters


Pharma Centrifuge Solutions

Therapeutic Antibodies

The use of monoclonal antibodies (mAb) has been accepted as an effective cure for the treatment of cancer and autoimmune diseases. Today antibodies dominate the biopharmaceutical market and their development and production using hybridoma cells is widely established. Rising time and cost pressures in combination with the demand for higher throughput make the development of therapeutic antibodies challenging.

Many factors have to be taken into account and alternative solutions are evaluated to battle challenges like sample protection, efficiency, and reproducibility. In addition, optimized ergonomics can reduce hands-on time, protect lab staff from work-associated injuries and improve overall performance.


Drug Discovery Workflow

The mAb drug discovery process includes several centrifugation steps with individual requirements for the used device. Eppendorf solutions of centrifuges, rotors and vessel adapters are based on protocol evaluation and customer interviews within mAb drug discovery community.

We offer a variety of comprehensive packages for R&D from our large centrifuge portfolio which best fit your needs - so you have the peace of mind to focus on the medicine of tomorrow.

 


Eppendorf Centrifuge Solutions

1. Molecular Biology Solution 1. Molecular Biology Solution

Centrifuge 5425 +Rotor FA-18x2-Kit

Target antigen production, sequencing sample preparation, and antibody humanization involve a great deal of repetitive molecular biology handling steps. This includes spin column based MiniPrep kits for nucleic acid purification, gel extraction, and PCR clean-up. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Microcentrifuges have entered a new generation, where the completely newly developed Centrifuge 5425 in combination with kit rotor specifically designed for 1.5/2.0 mL spin column kits.

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2. High Speed Solution 2. High Speed Solution

Centrifuge 5910 R + Rotor FA-6x50

Lysate clarification in nucleic acid purification protocols for target antigen production and antibody humanization include high speed centrifugation runs with up to 20,000 x g. High numbers of temperature-sensitive samples with up to 50 mL need to be processed. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Refrigerated benchtop Centrifuge 5910 R in combination with low-weight aluminum rotors offer advanced cooling performance for optimal sample protection and reproducibility.

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3. Big Volume Harvesting Solution 3. Big Volume Harvesting Solution

Centrifuge 5910 R + Rotor S-4xUniversal + 4 x 1l adapter + 4 x 1l bottle

The production of antibodies in cell lines and antigens in bacteria involve harvesting cells from large volume suspensions of several liters. To guarantee sample integrity, cooling is key in these process steps. Refrigerated benchtop Centrifuge 5910 R in combination with 4 liter swing-bucket Rotor S-4xUniversal offer advanced cooling performance for optimal sample protection and reproducibility. For improved sustainability and economic efficiency large-volume bottles are reusable, autoclavable, UV-resistant and dishwasher-proof.

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4. Versatility Solution 4. Versatility Solution

Centrifuge 5910 R + Rotor S-4xUniversal + 4 x 5/15/plate adapter + 4 x 50/250/plate adapter

Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Refrigerated benchtop Centrifuge 5910 R in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. For faster high-yield PBMC isolation, acceleration and deceleration ramps can be selected individually.

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5. High-Throughput Screening Solution 5. High-Throughput Screening Solution

Centrifuge 5920 R + Rotor S-4-1000 (plate/tube bucket) + 4 x plate carrier

High-throughput screening for the identification of antibody hits in culture supernatants of hybridoma clones is usually performed in microtiter plates (MTPs) with 96 or more wells. Various methods are required and all include multiple centrifugation steps of temperature-sensitive samples in MTPs. Refrigerated benchtop Centrifuge 5920 R in combination with swing-bucket Rotor S-4x1000 with plate/tube buckets offer super-high capacity of up to 28 MTPs per run and an advanced cooling performance for optimal sample protection.

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6. Low-Throughput Plate Solution 6. Low-Throughput Plate Solution

Centrifuge 5430 + Rotor A-2-MTP

For the selection and characterization of antibody leads low-throughput screening assays are performed in microtiter plates (MTPs). Various methods are required and all include multiple centrifugation steps. Centrifuge 5430 in combination with swing-bucket Rotor A-2-MTP offer an economical solution for two MTPs per run in a small footprint. Centrifuge 5430 also accommodate rotors for 15 mL and 50 mL conical tubes and high-capacity rotors for 1.5/2.0 mL tubes.

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7. Classic Cell Culture Solution 7. Classic Cell Culture Solution

Centrifuge 5810 + Rotor S-4-104 + 4 x 5/15 mL adapter + 4 x 50 mL adapter

In a final step of the mAb drug discovery workflow a stable cell line is established that can express high amounts of humanized monoclonal antibody long-term. Cultivation and selection of stable cell lines require multiple centrifugation steps. Centrifuge 5810 in combination with Rotor S-4-104 offer an economical solution with an excellent footprint to capacity ratio. This setup is ideal for standard cell culture applications including cell harvesting in 15 mL or 50 mL conical tubes.

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Discover Eppendorf Centrifuges in Videos

Since 1964 Eppendorf has been manufacturing high-quality centrifuges and, from 1991, its R&D and production facilities in Leipzig, Germany have played a major role in this ongoing success story. Modern production methods, professional warehousing, highly qualified employees and a strong commitment to sustainability provide the basis for state-of-the-art products with renowned Eppendorf quality.

Our 5910R Centrifuge supports your therapeutic antibody drug discovery workflow with three innovative universal adapters, which accommodate a selection of tubes, plates and bottles. This improves handling and saves time. Learn more about it in our video!


Discover the Flow

Screening assays, protein expression, cell culture and upscaling are the main tools utilised during mAb drug discovery. The diverse methodology involves various vessel formats, kit applications, cooled sample handling, high-throughput applications or cell handling. All equipment needs to meet user requirements to ensure continued efficiency, reliability, sample and user safety. So, we have tailored perfect centrifugation bundles to the specific requirements of each mAb workflow application!

Do you have less time but more samples? Antigen concentration, hybridoma development, flow cytometry, and PBMC isolation involve many centrifugation runs with diverse vessel formats and temperature-sensitive samples. Our solution helps you handle versatile samples trouble-free with universal rotors and adapters, optimal cooling for sample safety, and programmable settings for fast and reproducible operation.



Literature Resources


Products

Eppendorf™ Polypropylene DNA LoBind Polypropylene Microcentrifuge Tube

Maximize sample recovery of nucleic acids by significantly reducing sample-to-surface binding with these tubes. Eppendorf™ Polypropylene DNA LoBind Tubes are ideal for sample preparation and long-term storage. X250 DNA LoBind tube 0,5ml PCR clean

Eppendorf™ Polypropylene Protein LoBind Microcentrifuge Tube

Store peptide, antibody or virus samples in these LoBind microcentrifuge tubes. Eppendorf™ Polypropylene Protein LoBind Microcentrifuge Tubes are ideal for work in protein research, mass spectrometry and protein array sample preparation. x100 Protein LoBind tube 0,5ml, PCR clean

Eppendorf™ Polypropylene Graduated Microtubes

Use these tubes for safe handling of valuable and toxic samples as well as radioactive substances or DNA. Eppendorf™ Polypropylene Graduated Microtubes feature a hinged lid with a small hook that clips around the rim of the tube. x1000 Safe-Lock microtube 1.5ml, red

Eppendorf™ epTIPS™ Standard Pipettor Tips

Use these universal pipette tips with a wide variety of pipette models. Eppendorf™ epT.I.P.S.™ Standard Pipettor Tips feature optimal wetting properties, high transparency and special, certified purity grades. X1000 Pipette tips Eppendorf Standard 1250ul

Eppendorf™ epTIPS™ Pipettor Tip Reloads

Use this refill system for worry-free work in the molecular biology lab. Eppendorf™ epT.I.P.S.™ Pipettor Tip Reloads allow contamination-free handling of the refill trays. Plastic lids protect the trays from direct contact during transfer to the working box . X960 CE-IVD epTIPS Reloads 50-1250ul L 103mm, 10 stacked trays of 96 tips

Eppendorf™ Combitips advanced™ Standard Pipettor Tips

Use these tips for comfortable handling while preventing damage to gloves. Eppendorf Combitips advanced™ Standard Pipetter Tips are optimized to meet the increased requirements of a modern laboratory, and offered in three different purity grades to support any application. X100 Combitips advanced 0,2ml light blue bagged/25

Eppendorf™ 3810X Polypropylene Microcentrifuge Tube

Use these tubes for all applications in molecular biology, clinical chemistry and biochemistry. Eppendorf™ 3810X Polypropylene Microcentrifuge Tube offers comfort and dependability for sample preparation, centrifugation and storage. x1000 Microtube 3810x1.5ml green

Eppendorf™ Combitips Advanced™ Biopur™ Pipette Tips

Meet the increased requirements of a modern laboratory with these pipetter tips. Eppendorf™ Combitips Advanced™ Biopur™ Pipetter Tips make working with radioactive, aggressive or toxic materials safer. Hermetically sealed piston prevents aerosol contamination. X100 Combitips advanced 0,2ml, light blue, Biopurindividually wrapped

Eppendorf™ Microloader™ Pipette Tips

Use these extremely long, fine and flexible tips for filling of microcapillaries. Eppendorf ™ Microloader™ Pipette Tips are ideal for micro-injection and where additional reach is needed. X192 Tip Eppendorf Microloader 0.5ul to 20ul

Eppendorf™ Multipette™ M4 Dispenser with Holder

Use this pipette for viscous or foaming solutions and liquids with high vapor pressure through positive displacement principle. Eppendorf™ Multipette™ M4 Dispenser with Holder is the perfect instrument for completing long pipetting series. Multipette M4 w/holder (for wall mount and/orpipette carousel)

Eppendorf™ PCR Clean Colorless Safe-Lock Centrifuge Tubes

Protect your samples with these centrifuge tubes. Eppendorf™ PCR Clean Colorless Safe-Lock Centrifuge Tubes feature a hinged Eppendorf Safe–Lock lid for outstanding protection against unintentional opening during incubation and storage. X500 Tubes safe lock PCR clean 0.5mL Eppendorf

Eppendorf™ Research™ plus EU-IVD Single Channel Variable Volume Pipette

The ultra light Eppendorf Research plus pipette meets the highest needs in precision and accuracy – combined with ultimate ergonomics and increased flexibility. EU IVD Research plus G, single-channel, variable 2-20ul, light gray incl. epT.I.P.S. box EN10112444

Eppendorf™ Eppendorf Tubes™ Conical Tubes

Use these tubes for the simple and safe processing of sample volumes up to 5.0mL. Eppendorf Tubes™ represent the perfect option for working with medium–sized sample volumes. Eppendorf Tubes, 5mL, Starter Pack, PCR clean,

Eppendorf™ Pipette Carousel 2

Carries up to six manual single or multi-channel Eppendorf pipettes. Carousel 2 for 6 Research Plus or Reference or Biomaster pipettes

Eppendorf Tubes™ 3810X

Use these tubes for sample preparation, centrifugation or storage. Eppendorf Tubes™ 3810X fit perfectly into your workflows. x1000 Microtube 3810x1.5ml clear PCR Clean

Eppendorf™ Safe-Lock Tubes

The hinged lid of Eppendorf Safe-Lock Tubes offers outstanding protection against unintentional opening, and completely eliminates any danger of leaks during incubation and storage. x1000 Safe-Lock micro test tubes, 2ml, green

Eppendorf™ Repeater™ M4 Starter Kit

Use this dispenser for precise and accurate dispensing of viscous solutions, contamination-free dispensing of toxic, radioactive and other hazardous liquids. Eppendorf™ Repeater™ M4 Starter Kit is ideal for repetitive dispensing of volumes in long series such as tube or plate filling. Multipette M4 Starter Kit, w/Combitip Rack andCombitip Assortment Pack

Eppendorf™ Research plus™ Variable Adjustable Volume Pipettes: Single-Channel

Adapt for specific liquids or other external conditions with these ultra-light fully autoclavable pipettes. Eppendorf Research plus™ Variable Adjustable Volume Pipettes feature a spring loaded tip cone, a secondary adjustment option and an improved volume display. non-IVD Research plus G, single-channel, variable30-300ul, orange, incl. epT.I.P.S. box

Eppendorf™ UVette™

Use these cuvettes for measuring small volumes (≥50μL). The Eppendorf™ UVette™ is a disposable UV/Vis cuvette with a light transmission of 220 to 1,600nm. UVettes offer superb purity, convenience and precision for photometric analysis. x80 UVette

Multipette™ E3 Single Channel Pipet

Dispenses and pipets any liquid regardless of viscosity while maintaining the highest standards for accurate results. Eppendorf™ Multipette™ E3 Single Channel Pipet provides full volume control, improved accuracy, and reproducibility while reducing physical strain during use. Multipette E3, with universal charging adapter, charger stand, 2 Combitips advanced assortment packs

Eppendorf™ ThermoMixer™ C, Basic Device without Thermoblock

Use this temperature control device for heating and cooling almost any of your lab vessels. The Eppendorf ThermoStat™ C is the ideal device to accurately set and maintain temperatures. ThermoMixer C w/o thermoblock, 220-240V GB plug

Eppendorf™ epTIPS™ Pipette Tip Reloads

Use this refill system for worry-free work in the molecular biology lab. Eppendorf™ epT.I.P.S.™ Pipette Tip Reloads allow contamination-free handling of the refill trays. Plastic lids protect the trays from direct contact during transfer to the working box. X960 CE-IVD epTIPS Reload 0.1-20ul PCR-clean racked/96

Eppendorf™ Autoclavable Safe-Lock Micropestle

Use this pestle for resuspending pellets. Eppendorf™ Autoclavable Safe-Lock Micropestles feature two sides for optimal fit in 1.5 and 2.0mL vessels. X10 Micropestle autoclavable for 1.5 and 2.0mL

Eppendorf™ Minispin™ Benchtop Centrifuge

Save space with these microcentrifuges. Eppendorf™ Minispin™ Benchtop Centrifuges feature a metal rotor, inner centrifuge lid and lid latch for maximum operation safety. Optimal ventilation flow reduces heating and protects temperature-sensitive samples CE-IVD Centrifuge Minispin, GB-plug, with 12x2ml rotor

Eppendorf™ Refrigerated Centrifuge with Rotor

Designed for separating liquid substance mixtures with different densities and analyzing samples from human body in in-vitro diagnostic applications. CE-IVD Cooled Centrifuge 5804R G, 230V, GB-plug, w/ rotor A-4-44 and 15/50ml adapters

Eppendorf™ Xplorer™ 12-Channel Electronic Pipette

Designed to aspirate and dispense liquid volumes from 0.5µL to 10mL while eliminating many of potential error risks associated with manual pipetting. Eppendorf™ Xplorer™ 12-Channel Electronic Pipettes feature intuitive operating concept and ergonomic design. Xplorer, 12-channel, 50-1200ul, green

Eppendorf™ Concentrator Plus with Pump and GB Plug

Provide best treatment for sample and assure quick, efficient and gentle vacuum concentration of DNA/RNA, nucleotides, proteins and other liquid or wet samples. Eppendorf™ Concentrator Plus Complete is an ergonomic, intuitive and durable solution that fits on your lab bench. Concentrator Plus, complete w/pump, 230V GB-plug,w/o rotor

Eppendorf™ Research™ Plus Pipetters

Use these pipettes for stress-free work procedures and intuitive operation. Eppendorf™ Research™ plus Pipettes meet the highest needs in precision and accuracy – combined with ultimate ergonomics and increased flexibility. non-IVD Research plus G, 3-pack, option 3, 100-1000ul/0,5-5mL/1-10mL, incl. epT.I.P.S. boxes

Eppendorf™ A-2-MTP Rotor

Compatible with Eppendorf™ 5430/5430 R Centrifuges. Eppendorf™ A-2-MTP Rotor features windshield design for whisper quiet operation. Rotor A-2-MTP w/2xMTP-buckets and rotor lidplus windshield

Eppendorf™ Safe-Lock Tubes

The hinged lid of Eppendorf Safe-Lock Tubes offers outstanding protection against unintentional opening, and completely eliminates any danger of leaks during incubation and storage. x500 Safe-Lock microtube 0.5ml, red

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High Capacity Shaker

Increase Your Yield with the Innova S44i Shaker

Are you looking for a stackable incubator shaker for particularly high protein expression yields in bacteria or yeast to quickly reach your ambitious targets? The Innova S44i from Eppendorf offers high vessel capacity and can sustain high speeds and loads 24/7.

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Shake faster! Ready for current high yield expression protocols

Current microbiological DNA and protein expression protocols lead to optimised yields with specialised flasks at shaking speeds of up to 400 rpm (e.g. Ultra Yield flasks from Thomson Instrument). Compared to other shakers in the market, the Innova S44i can provide and sustain high speeds at an orbit of 2.5 cm (1 in) 24/7 with maximum platform loads. This makes the Innova S44i a real workhorse for protein production. You can further increase your yield per footprint by triple stacking the device.

Eppendorf_863947

Shake more! Up to twice as many flasks as other shakers in the market

Platform, chamber, and the heavy duty Eppendorf X-drive of the Innova S44i are optimised for high vessel capacity and load – to maximise the throughput of your lab. If vessel capacity is crucial for you, compare the capacity of the Innova S44i with other shakers in the market. Of course, the Innova S44i is also compatible with online in-flask monitoring systems like CGQ (by aquila biolabs) or PreSens, and microplate-screening systems like the Duetz-System.

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Resources

WhatsNext

Discover Eppendorf WhatsNext Promotion

Enter promotion code 15623 to access the promotional prices until 30th June 2021.


Enhance Your Lab

Expand your application areas with our exclusive offer.

Save 20% on the Centrifuge 5425 R

Increase Your Throughput

Save multiple centrifugation steps of temperature sensitive samples in MTPs.

Save 20% on the Centrifuge 5920 R

Get Started

Save time with our innovative 25mL conical tube with SnapTec cap.

Save 25% on the 25mL conical tube

Mix, Heat and Cool for High Flexibility

Reproducible and standardised thawing of your valuable cell samples.

Save 20% on the Eppendorf ThermoMixer C

Amplify Your PCR

Maximum flexibility across a variety of block versions, including eco units.

Save 25% on the Mastercycler Nexus

Faster Finish Means Faster Publish

With Move It™ you can easily save up to 70% of your time.

Exchange Your Old Pipettors

Bundle Savings

More efficiency with extra capacity. Reduce your effort with Innova S44i.

Save 25% on the Innova S44i Premium Incubator

Don’t Waste Time Cleaning

The New Brunswick™ S41i CO₂ incubator shaker will support your protein expression.

Save 25% on the New Brunswick S41i Incubator