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Products

IBA Lifesciences™ Columna de flujo por gravedad de 5 ml

5 ML GRAVITY FLOW STREP-TACTIN(R) SEPHAROSE COLUMN, 5 mL 00 Store in 4C Fridge

IBA Solutions for Life Science™ D-destiobiotina

Used for elution of Strep-tag™ II proteins from Strep-Tactin™ 1 GR D-DESTHIOBIOTIN 1G STORE IN 4C FRIDGE

IBA Lifesciences™ Separador magnético

Ayuda a la purificación rápida de proteínas Strep-tag™. Magnetic Separator for 24 rxntubes, 1piece

IBA GmbH™ Kit de aislamiento MB CD4+ CD25+ Treg Fab Streptamer™

El kit de aislamiento MB CD4+ CD25+ Treg Fab Streptamer™ IBA GmbH™ se emplea para el aislamiento celular reversible que proporciona células diana sin etiqueta. CD4+ CD25+ Treg Fab Streptamer Isolation Kit MB

IBA Solutions for Life Science™ Tampón de d-destiobiotina E

25 ML D-DESTHIOBIOTIN, 25MM BUFFER SOLUTION (10XBuffer E) contains Tris pH 8, NaCl and EDTA 25mL

IBA GmbH™ Kit de aislamiento de micropartículas magnéticas de Treg CD4+ CD25+ CD45RA+ para Fab Streptamer™

El kit de aislamiento de micropartículas magnéticas de Treg CD4+ CD25+ CD45RA+ para Fab Streptamer™ IBA GmbH™ está diseñado para el aislamiento celular reversible al proporcionar células objetivo sin etiquetas. CD4+ CD25+ CD45RA+ Treg Fab Streptamer Isolation Kit MB

IBA Solutions for Life Science™ Tampón de regeneración 10X Strep-tag™

100 ML STREP-TAG(R) REGENERATION BUFFER WITH HABA(10x Buffer R) 100mL Store in 4C Fridge

IBA Solutions For Life Science™ Gravity Flow Strep-Tactin Sepharose 4FF

Column, cartridge and plate formats for Strep-Tactin™ resins 10 ML GRAVITY FLOW STREP-TACTIN(R) SEPHAROSEcolumn, 10 mL 00 Store in 4C Fridge

IBA GmbH™ E. coli Expression Vector with Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA GmbH™ E. coli Expression Vector with Secretion allows for the expression of proteins in E.coli. pASG-IBA4; 5µg

IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination. pLSG-IBA167; 5µg

IBA GmbH™ CD8 Fab-TACS™ Gravity Kit

IBA GmbH™ CD8 Fab-TACS™ Gravity Kit is for reversible cell isolation delivering label-free target cells. CD8 Fab-TACS Gravity Isolation Kit,Human single marker,4 columns,capacity 50ml whole blood each

IBA GmbH™ Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA43; 5µg

IBA GmbH™ Small Mammalian Expression Vector

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA GmbH™ Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system. pDSG-IBA43; 5µg

IBA GmbH CD4 Isolation Kit for FABian&trade, Mouse

For automated positive cell separation. The Isolation Kit for FABian&trade provides label-free target cells without the use of high affinity antibodies and magnetic beads CD4 Isolation Kit for FABian®, mouse, 1 Kit

IBA GmbH™ E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA GmbH™ E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli. pASG-IBA23; 5µg

IBA GmbH™ Mammalian Expression Vector with Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBAwt2; 5µg

IBA Solutions for Life Science™ D-Biotina

2 GR D-Biotin (not for Strep-tag applications) 2g

IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter. pYSG-IBA45; 5µg

IBA Lifesciences™ Tampón bloqueante de biotina

El tampón bloqueante de biotina se recomienda para bloquear proteínas biotiniladas en Western blots antes de la detección de proteínas Strep-tag con el conjugado Strep-Tactin AP o conjugado Strep-Tactin HRP. 2 ML BIOTIN BLOCKING BUFFER 2ML STORE IN 4C FRIDGE

IBA GmbH™ Adaptador de gravedad Fab-TACS™

Fisher Scientific - El adaptador de gravedad Fab-TACS™ IBA GmbH™ está diseñado para usarse en un aislamiento celular reversible que aporte células diana sin etiqueta. Fab-TACS Gravity Adapter, allows use of gravity columns with 50ml tubes

IBA Solutions For Life Science™ Strep-tag II Peptide

Buffers and reagents for Strep-tag™ 1.8 MG Strep-tag(R) II Peptide, 1.8 mg 00 Store

IBA Solutions For Life Science StarGate™ pESG-IBA Acceptor Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen. pESG-IBA45; 5µg

IBA GmbH CD45RA Isolation Kit for FABian™

For automated positive cell separation. The Isolation Kit for FABian&trade provides label-free target cells without the use of high affinity antibodies and magnetic beads CD45RA Isolation Kits for FABian(R), human, 10 Kit

IBA GmbH™ Strep-Tactin™XT Superflow™ High Capacity

IBA GmbH™ Strep-Tactin™XT Superflow™ High Capacity Reliable is a one-step purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins with high affinity and unsurpassed purity. 50ml Strep-Tactin® XT Superflow® high capacity

IBA GmbH Suspensión de proteína A agarosa al 50 %

Proteína A agarosa para la purificación de anticuerpos monoclonales y policlonales mediante cromatografía de afinidad Protein A Agarose 50% suspension; 1 ml

IBA Solutions For Life Science StarGate™ pPSG-IBA Acceptor Vectors for E. coli Expression

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins. pPSG-IBA164; 5µg

IBA Solutions for Life Science™ Células competentes de «E. coli» TOP10

Las células son muy eficientes y son ideales para las aplicaciones Stargate™ Competent E. coli TOP 10 cells, 20 reactions 00

IBA GmbH™ Patrón de conjunto de soluciones Streptamer™

El patrón de conjunto de soluciones IBA GmbH™ Streptamer™ está diseñado para su uso en el lavado y la disociación. Streptamer solution set standard, buffer 50ml,Biotin Stock 1ml

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Featured Products

Immobilisation with Near Covalent Binding Affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR Experiments

  • Indirect immobilisation of the ligand prevents the ligand to be inactivated or influenced by the immobilisation procedure
  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated by an established procedure
  • Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag:Strep-Tactin XT interaction

Try our New High Affinity Strep-tag™ Technology


Try our new high affinity Strep-tag® system

Strep-Tactin XT 4Flow™ Starter Kit consists of:

  • Strep-Tactin XT 4Flow gravity flow column 
  • Buffer W (Strep-Tactin/Strep-Tactin XT Wash Buffer) 
  • Buffer BXT (Strep-Tactin XT Elution Buffer with Biotin) 
  • Buffer XT-R (Strep-Tactin XT Regeneration Buffer with Biotin MgCl2)
  • Strep-Tactin conjugated with HRP (for Western blot detection)
  • Short protocol

Exosome Isolation - Quick and Simple


Try our new high affinity Strep-tag® system

The Fab-TACS™ Exosome Isolation Technology offers:

  • Highly specific and reproducible isolations with minimal contamination in a source-independent manner
  • Quick and simple protocol, without magnetic beads or ultracentrifugation, no extra device needed
  • Label-free exosomes with intact biological functions due to reversible reagents
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free, non-activated cells
  • From whole blood, buffy coat, PBMCs or murine samples
  • Manual or automated cell separation

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Bead-free

Peptides for T cell stimulation

  • Purity > 85 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ and of receptor-specific T cells with Fab Streptamers™

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning


Cloning & Transfection

More than 150 Twin/Strep-tag™ II expression vectors

  • Variety of Twin/Strep-tag II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors

Mammalian Expression


Cloning & Transfection

The MEXi mammalian expression system

Optimised for efficient protein expression and Strep-tag purification in mammalian cells.

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors from IBA with different features

Protein Purification


Protein Purification

Strep-tag™ technology from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA

Two different detection systems are provided:

  • Strep-Tactin and Strep-Tactin XT conjugate for Twin/Strep-tag II proteins
  • Monoclonal antibodies against Twin/Strep-tag II

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Exosomes

Traceless Affinity Exosome Isolation


Backbone Modifications

Quick and simple isolation of unlabelled exosomes using Fab-TACS™ technology

The Fab-TACS technology for exosome isolation offers

  • Highly specific and reproducible isolations
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Resources Magnetic Beads Sample Request
IBA_about_us

About IBA GmbH

Solutions For Life Sciences

IBA Lifesciences provides innovative technologies for life science applications. The product portfolio includes tools for cell selection and expansion as well as protein production and assays.