IBA Lifesciences

IBA Lifesciences GmbH
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Featured Products

Immobilization with Near Covalent Binding Affinity


Immobilization with near covalent binding affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for Bio-Layer Interferometry (BLI) or Surface Plasmon Resonance (SPR) experiments

  • Indirect immobilization of the ligand prevents it from inactivation or influences of the immobilization procedure
  • High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
  • Ligands can be captured directly from complex samples
  • Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip

Our Perfect Tool for Isolation of Proteins or Complexes


Try our new high affinity Strep-tag® system

MagStrep 'Type 3' XT Magnetic Beads offer:

  • High specificity and low background resulting in high protein purity
  • High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
  • Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
  • Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows

Efficient Purification Even of Large Proteins


Try our new high affinity Strep-tag® system

Strep-Tactin™ XT 4Flow™ Starter Kit consisting of everything you need for a quick start:

  • Strep-Tactin™ XT 4Flow™ gravity flow column
  • All buffers required for washing, elution and regeneration
  • Strep-Tactin™ conjugated with HRP (for Western blot detection)
  • Short protocol

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins


Try our new high affinity Strep-tag® system

Applicable for purification, detection and immobilization of biotinylated molecules

Cells
IBS's Cell Selection

TACS - Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free, non-activated cells due to fully reversible binding
  • From whole blood, buffy coat, PBMCs or murine samples
  • Affinity chromatographic, magnetic or fluorescent cell separation

MHC I Streptamer™


Magnetic Cell Selection

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™ 

  • Label-free cell isolation according to antigen specificity
  • High specificity, sensitivity and reproducibility
  • From single cell suspensions (e.g., PBMCs or murine samples)
  • Affinity chromatographic, magnetic or fluorescent cell separation

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3: CD28 Streptamers™

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3: CD28 ratio 
  • Bead-free

Peptides for T-cell stimulation

  • Purity >85%
  • Endotoxin-free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Proteins
Protein

Cloning


Cloning & Transfection

More than 150 Twin-Strep-tag™/Strep-tag™ II expression vectors

  • Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors

Mammalian Expression


Cloning & Transfection

The MEXi mammalian expression system

Optimised for efficient protein expression and Strep-tag™ purification in mammalian cells.

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors from IBA with different features

Protein Purification


Protein Purification

Strep-tag™ technology from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (>95%)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA

Two different detection systems are provided:

  • Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
  • Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Exosomes

Surface-marker Specific Exosome Isolation


Backbone Modifications

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

  • Highly specific and reproducible isolation
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Resources
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About IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. The product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.