IBA Lifesciences GmbH

IBA Lifesciences GmbH
Products
IBA Lifesciences Strep-Tactin™ Conjugate

IBA Lifesciences Strep-Tactin™ Conjugate

Strep-Tactin™ conjugates with AP or HRP for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins 500 UL Strep-Tactin(R) AP conjugate 0.5mL Store

IBA Lifesciences 10x Buffer BXT

IBA Lifesciences 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT. 25ML 10x Buffer BXT; Strep-TactinXT Elution Buffer

Anhydrotetracycline, IBA Lifesciences

Anhydrotetracycline, IBA Lifesciences

CAS: 1665-56-1 Molecular Formula: C22H22N2O7 Molecular Weight (g/mol): 426.43 InChI Key: JGRMImmCZBOWCW-LCGGBVFXSA-N Synonym: anhydrotetracycline, 5 A,6-anhydrotetracycline, 4 s-anhydrotetracycline, cxcveerymjzmmm-docrcchosa-n, 4 s,4 AS,12 AS-4-dimethylamino-3,10,11,12 A-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4 A,5,12,12 A-hexahydrotetracene-2-carboxamide PubChem CID: 54675758 50 MG ANHYDROTETRACYCLINE 50MG STORE IN -20CFreezer

IBA Lifesciences Strep-Tactin™ Sepharose™ Resin

IBA Lifesciences Strep-Tactin™ Sepharose™ Resin

Strep-Tactin™ Sepharose™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 1 LT Strep-Tactin(R) Sepharose 50 % suspension 1L

IBA Lifesciences MagStrep 'Type3' XT Beads

IBA Lifesciences MagStrep 'Type3' XT Beads

MagStrep 'type3' XT beads are magnetic beads (5% suspension) to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 10ML MagStrep "type3" XT beads 5% suspension

IBA Lifesciences Desthiobiotin

IBA Lifesciences Desthiobiotin

Desthiobiotin powder for preparation of Strep-Tactin™ elution buffer 0.107 GR D-DESTHIOBIOTIN, 0.107G FOR 200ML 1Xbuffer 00 Store in 4C Fridge

IBA Lifesciences Magnetic Separator

IBA Lifesciences Magnetic Separator

The Magnetic Separator is a device for separation of magnetic beads from residual solution Magnetic Separator for 24 rxntubes, 1piece

IBA Lifesciences 10x Buffer W - Wash Buffer

IBA Lifesciences 10x Buffer W - Wash Buffer

Strep-Tactin™ and Strep-Tactin™XT wash buffer 100 ML Strep-tag(R) washing buffer (10x buffer W)

IBA Lifesciences StrepMAB-Classic

IBA Lifesciences StrepMAB-Classic

Monoclonal antibody to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins 100 UG STREPMAB-CLASSIC, STREP-TAG II SPECIFICmonoclonal antibody, mouse/IgG1, purified 100µg

IBA Lifesciences Biotin

IBA Lifesciences Biotin

2 GR D-Biotin (not for Strep-tag applications) 2g

IBA Lifesciences Strep-Tactin™ Coated Microplate

IBA Lifesciences Strep-Tactin™ Coated Microplate

Strep-Tactin™ coated microplate is a ready-to-use microplate for assay applications Strep-Tactin® coatedmicroplate, 1 plate, 1plate

IBA Lifesciences 10x Buffer E - Elution Buffer

IBA Lifesciences 10x Buffer E - Elution Buffer

Strep-Tactin™ elution buffer with desthiobiotin 25 ML D-DESTHIOBIOTIN, 25MM BUFFER SOLUTION (10XBuffer E) contains Tris pH 8, NaCl and EDTA 25mL

IBA Lifesciences Protein A Agarose Resin

IBA Lifesciences Protein A Agarose Resin

Protein A Agarose resin is a 50% suspension to purify monoclonal and polyclonal antibodies Protein A Agarose 50% suspension; 200 ml

IBA Lifesciences Strep-Tactin™XT Spin Column Kit

IBA Lifesciences Strep-Tactin™XT Spin Column Kit

IBA Lifesciences GmbH Strep-Tactin™XT Spin Column Kit allows for rapid parallel purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins. 1 KIT Strep-Tactin XT Spin Column Kit

IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Resin

IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Resin

Strep-Tactin™ Superflow™ High Capacity Resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 50 ML Strep-Tactin(R) Superflow(R) high capacity,50% suspension 50mL Store in 4C Fridge

IBA Lifesciences 10x Buffer R - Regeneration Buffer

IBA Lifesciences 10x Buffer R - Regeneration Buffer

10x Buffer R is a Strep-Tactin™ regeneration buffer with HABA 100 ML STREP-TAG(R) REGENERATION BUFFER WITH HABA(10x Buffer R) 100mL Store in 4C Fridge

IBA Lifesciences GFP-Strep-tag control protein

IBA Lifesciences GFP-Strep-tag control protein

GFP-Strep-tag control protein for Strep-Tactin™ and Strep-Tactin™XT applications GFP-Strep-tag control protein

IBA Lifesciences MART-1 peptide ELAGIGILTV (HLA-A*0201)

IBA Lifesciences MART-1 peptide ELAGIGILTV (HLA-A*0201)

Single peptide synthesized as presented by the MHC class I allele 1MG MART-1 peptide ELAGIGILTV (HLA-A*0201)

IBA Lifesciences Strep-Tactin™ Superflow™, 50% Suspension

IBA Lifesciences Strep-Tactin™ Superflow™, 50% Suspension

Used for purification of recombinant Strep-tag™ proteins under gravity flow and low pressure or FPLC 1 LT Strep-Tactin(R) Superflow(R), 50 % suspension1L Store in 4C Fridge

IBA Lifesciences Strep-Tactin™ Sepharose™ Column

IBA Lifesciences Strep-Tactin™ Sepharose™ Column

Strep-Tactin™ Sepharose™ column is a Gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 1 ML Gravity flow Strep-Tactin(R) Sepharose column, 1 mL 00 Store in 4C Fridge

IBA Lifesciences Competent E. coli TOP10

IBA Lifesciences Competent E. coli TOP10

Competent E. coli TOP10 are chemically competent cells for plasmid propagation Competent E. coli TOP 10 cells, 20 reactions 00

IBA Lifesciences StrepMAB-Immo

IBA Lifesciences StrepMAB-Immo

StrepMAB-Immo is a monoclonal antibody to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins 100 UG STREPMAB-IMMO, MURINE HIGH AFFINITY STREP-Tag II specificmonoclonal IgG1, purified 100µg

IBA Lifesciences CMV pp65 peptide NLVPMVATV (HLA-A*0201)

IBA Lifesciences CMV pp65 peptide NLVPMVATV (HLA-A*0201)

Single peptide synthesized as presented by the MHC class I allele 1MG CMV pp65 peptide NLVPMVATV (HLA-A*0201)

IBA Lifesciences Influenza M peptide GILGFVFTL (HLA-A*0201)

IBA Lifesciences Influenza M peptide GILGFVFTL (HLA-A*0201)

Single peptide synthesized as presented by the MHC class I allele 1MG Influenza M peptide GILGFVFTL (HLA-A*0201)

IBA Lifesciences Twin-Strep-tag™ Capture Kit

IBA Lifesciences Twin-Strep-tag™ Capture Kit

The Twin-Strep-tag™ Capture Kit can be used for reversible capture of Twin-Strep-tag™ fusion proteins for SPR analyses Twin-Strep-tag capture kit, for coating a BiacoreCM5 sensor chip with Strep-Tactin XT

IBA Lifesciences StrepMAB-Classic Conjugate

IBA Lifesciences StrepMAB-Classic Conjugate

StrepMAB-Classic conjugates are monoclonal antibodies with different conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins 50UG StrepMAB-Classic DY-649 conjugate

IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Column

IBA Lifesciences Strep-Tactin™ Superflow™ High Capacity Column

Gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins X5 Gravity flow Strep-Tactin(R) Superflow(R) high

IBA Lifesciences CD4 Fab-Strep

IBA Lifesciences CD4 Fab-Strep

Low affinity Fab fragment fused to a Twin-Strep-tag™ 50UG Low affinity Fab fragment fused to a Twin-Strep-tag and specific for human CD4

IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. E. coli Expression Vector with No Secretion allows for the expression of proteins in E. coli. pASG-IBA168; 5µg

IBA Lifesciences Strep-Tactin™ MacroPrep™ Resin

IBA Lifesciences Strep-Tactin™ MacroPrep™ Resin

Strep-Tactin™ MacroPrep™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins 20 ML Strep-Tactin(R) MacroPrep(R), 50 % suspensio

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Featured Products

Immobilisation with Near Covalent Binding Affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR Experiments

  • Indirect immobilisation of the ligand prevents the ligand to be inactivated or influenced by the immobilisation procedure
  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated by an established procedure
  • Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag:Strep-Tactin XT interaction

Try our New High Affinity Strep-tag™ Technology


Try our new high affinity Strep-tag® system

Strep-Tactin XT 4Flow™ Starter Kit consists of:

  • Strep-Tactin XT 4Flow gravity flow column 
  • Buffer W (Strep-Tactin/Strep-Tactin XT Wash Buffer) 
  • Buffer BXT (Strep-Tactin XT Elution Buffer with Biotin) 
  • Buffer XT-R (Strep-Tactin XT Regeneration Buffer with Biotin MgCl2)
  • Strep-Tactin conjugated with HRP (for Western blot detection)
  • Short protocol

Exosome Isolation - Quick and Simple


Try our new high affinity Strep-tag® system

The Fab-TACS™ Exosome Isolation Technology offers:

  • Highly specific and reproducible isolations with minimal contamination in a source-independent manner
  • Quick and simple protocol, without magnetic beads or ultracentrifugation, no extra device needed
  • Label-free exosomes with intact biological functions due to reversible reagents
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free, non-activated cells
  • From whole blood, buffy coat, PBMCs or murine samples
  • Manual or automated cell separation

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Bead-free

Peptides for T cell stimulation

  • Purity > 85 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ and of receptor-specific T cells with Fab Streptamers™

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning


Cloning & Transfection

More than 150 Twin/Strep-tag™ II expression vectors

  • Variety of Twin/Strep-tag II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors

Mammalian Expression


Cloning & Transfection

The MEXi mammalian expression system

Optimised for efficient protein expression and Strep-tag purification in mammalian cells.

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors from IBA with different features

Protein Purification


Protein Purification

Strep-tag™ technology from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA

Two different detection systems are provided:

  • Strep-Tactin and Strep-Tactin XT conjugate for Twin/Strep-tag II proteins
  • Monoclonal antibodies against Twin/Strep-tag II

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Exosomes

Traceless Affinity Exosome Isolation


Backbone Modifications

Quick and simple isolation of unlabelled exosomes using Fab-TACS™ technology

The Fab-TACS technology for exosome isolation offers

  • Highly specific and reproducible isolations
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Resources
IBA_about_us

About IBA Lifesciences GmbH

Solutions For Life Sciences

IBA Lifesciences GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection and expansion as well as protein production and assays.