Invitrogen™ Texas Red Polyclonal Antibody

Product Code.	Quantity	unitSize
10194282	500 μL	Each

Product Code. 10194282 Supplier Invitrogen™ Supplier No. A6399

Description

Rabbit Polyclonal Antibody

We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence. These anti-dye antibodies, including those that recognize the tetramethylrhodamine (TRITC) and Texas Red™ fluorophores, can also serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes. Our anti-tetramethylrhodamine (A6397) and anti-Texas Red (A6399) antibodies have been raised against the tetramethylrhodamine and Texas Red fluorophores, respectively. These antibodies quench much of the fluorescence of the complementary dyes. However, due to the related chemical structures, the anti-tetramethylrhodamine antibody crossreacts with the Texas Red fluorophore and the anti-Texas Red antibody cross-reacts with tetramethylrhodamine. Also, both antibodies tightly bind the Rhodamine Red™ fluorophore. Thus, either antibody can serve as an effective probe for tetramethylrhodamine, Texas Red, or Rhodamine Red dyes. We use a sensitive quenching assay to ensure that this antibody is provided at a consistently high titer value. As supplied, 20 μL of the antibody solution is certified to produce ≥50% of the maximal fluorescence quenching of 1 mL of a 50 nM solution of Texas Red dye, assayed in 100 mM sodium phosphate, pH 8.0. Maximal quenching for Texas Red is ~60% of the fluorescence of the free dy...

Unlike biotin, which is an endogenous ligand in mitochondria, dye-based haptens permit background-free staining of cells and tissues and a sensitive alternative to biotin-streptavidin methods.

Specifications

• Antigen: Texas Red
• Applications: Immunohistochemistry, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5mM sodium azide; pH 7.2
• Gene Alias: C31H29ClN2O6S2; Sulforhodamine
• Host Species: Rabbit
• Immunogen: Texas Red.
• Purification Method: Purified
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG
• Target Molecule: Chemical