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Applied Biosystems™ TaqMan™ RNA-to-CT™ One-Step Kit

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

Brand:  Applied Biosystems™ 4392656

Code : 50

Additional Details : Weight : 0.00450kg

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Product Code. 10045714

  • £3865.00 / 1 set

EU Stock 16

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

  • Accurate across a wide dynamic range for dependable performance
  • High sensitivity, enabling low copy number detection and precise quantification
  • Validated with TaqMan™ Gene Expression Assays for easy experimental setup
  • Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
  • Fewer pipetting steps means less hands-on-time and less chance for errors

Efficiency across a Broad Range of Input Concentrations
  • Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities
  • The result is comparable to that of the TaqMan™ RNA-to-CT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan™ One-Step RT-PCR Master Mix Reagents.

High Sensitivity at Low Target Concentration
  • The sensitivity of TaqMan™ RNA-to-CT™ One-Step Kit was validated using a synthetic RNA template for which copy number is precisely known
  • Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates
  • Statistical analysis indicates high confidence of sample quantification based on a T-test, consistent with as few as 10 copies of target.

Order Info

Shipping Conditions: Wet Ice

Specifications

Specifications

Primer-Probe Compatability: TaqMan™
Passive Reference Dye: ROX (Pre-mixed)
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
AmpliTaq Gold™ DNA Polymerase
RNA
2000 Reactions
Primer-Probe Detection
Real time PCR (qPCR), real time PCR-based gene expression profiling
Sufficient for 2000 reactions at 50μL reaction volume. Includes: 10 x 5mL tubes of 2X master mix containing AmpliTaq Gold™ DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components (Store at 2° to 8°C after opening). 10 x 250μL tubes of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor (Store at -15° to -30°C).
UP (Ultra Pure)
Standard
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.