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Applied Biosystems™ TaqMan™ Protein Expression Assay Kit (hCSTB)
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Quantity:
100 reactions
Unit Size:
Each
Description
Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assay Kit (Human CSTB) contains the assay probes and a dilution buffer for the labelling of human cystatin-B protein (hCSTB) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hCSTB protein. Key features of the TaqMan™ Protein Expression Assay:
Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry
Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
Specifications
Specifications
| Content And Storage | Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C. |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | Low |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Fast DNA Polymerase |
| Reaction Speed | Fast |
| Technique | Proximity Ligation Assay |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Green Features | Sustainable packaging |
| Label or Dye | FAM |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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