Applied Biosystems™ TaqMan™ Protein Expression Assay Kit (hCSTB)

Artikelnummer. 10045894

Marke: Applied Biosystems™ 4405465

Beschreibung

TaqMan™ Protein Expression Assay Kit (hCSTB)

Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assay Kit (Human CSTB) contains the assay probes and a dilution buffer for the labelling of human cystatin-B protein (hCSTB) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hCSTB protein. Key features of the TaqMan™ Protein Expression Assay:

Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry

Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform

Allows greater sensitivity and a more quantitative readout than western blots and immunostaining

Spezifikation

• Content And Storage: Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C.
• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• PCR Method: qPCR
• Polymerase: AmpliTaq Fast DNA Polymerase
• Reaction Speed: Fast
• Technique: Proximity Ligation Assay
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Green Features: Sustainable packaging
• Label or Dye: FAM
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: TaqMan
• Product Type: Protein Expression Assay Kit
• Purity or Quality Grade: UP (Ultra Pure)
• Quantity: 100 reactions
• Shipping Condition: Wet Ice
• Sufficient For: 100 Reactions
• Target Specificity: Target-Specific
• Hot Start: No
• No. of Reactions: 100 reactions
• Sample Type: Cells

For Research Use Only. Not for use in diagnostic procedures.