TaqMan™ TaqMan™ MicroRNA Reverse Transcription Kit

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

Brand:  Applied Biosystems™ 4366596

Product Code. 10146854

Description

A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Components of this kit are used with the RT primer provided with the TaqMan™ MicroRNA Assay to convert miRNA to cDNA.

Order Info

Shipping Conditions: Dry Ice

Specifications

• Content And Storage: Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/μL), and MultiScribe™ RT enzyme (50U/μL) for 200 reactions. Store at -15 to -25°C.
• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Optimal Reaction Temperature: 42°C
• Product Type: MicroRNA Reverse Transcription Kit
• Shipping Condition: Dry Ice
• For Use With (Application): miRNA analysis
• Fidelity (vs. Taq): 10 X
• No. of Reactions: 200 Reactions
• Sample Type: RNA

• Detection Method: Primer-probe
• PCR Method: 2-step RT-qPCR
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
• Product Line: TaqMan™
• Reverse Transcriptase: MultiScribe™
• Sufficient For: 200 Reactions
• Concentration: 10X
• Final Product Type: cDNA
• Reaction Format: Separate Components
• Size (Final Product): 200 bp or less

For Research Use Only. Not for use in diagnostic procedures.