Merck Millipore RIPA Lysis Buffer, 10X

For use in immunoprecipitation and Western blotting. RIPA Lysis Buffer, 10X is a concentrated lysis buffer that has been filtered through a 0.2μm membrane and packaged aseptically.

Brand:  Merck Millipore 20-188

Product Code. 10014424

Description

Protein extraction and purification represent the first of many challenges in obtaining a quality lysate or purified protein sample that delivers publication-quality Western blot results. Merck quality reagents unite superior performance with speed to reduce exposure of proteins to unfavorable conditions, leading to more stable, intact proteins for downstream analysis.

• Use: For use in mammalian cell lysis
• Dilute one part 10X RIPA Lysis Buffer with nine parts sterile, distilled water
• Stir mixture and refrigerate at least two hours prior to use
• Immediately prior to use, add the protease and phosphatase inhibitors
• Filtered through 0.2μm membrane, packaged aseptically

Safety and Handling

Product Identifier

• RIPA Lysis Buffer, 10X

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash skin thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contain: hydroxy-poly(oxy-1,2-ethanediyl) branched, deoxycholic acid