Merck Millipore Proteinase K

From Tritirachium album

Brand:  Merck Millipore 1245680500

Product Code. 10036570

Description

• Chromatographically purified lyophilized 30 m Anson-U/mg
• For biochemistry and molecular biology EC 3.4.21.14
• Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
• Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
• Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
• Determination of enzyme localization on membranes
• Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
• Digestion of proteins from brain tissue samples for prions in Transmissible
• Spongiform Encephalopathies (TSE) research.
• Proteinase K is a stable and highly reactive serine protease.
• Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴).
• It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature.
• In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Safety and Handling

Product Identifier

• PROTEINASE K CHROMATOgrAPHICALLY PURIFIED, 500mg

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash skin thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.

Supplemental information

• MIXTURE LIST-Contain: proteinase k