PRKCN (PKD3) Recombinant Human Protein

PRKCN (PKD3) Recombinant Human Protein

Brand:  Invitrogen PV4044

Product Code. 10138782

Description

The PKC family of serine⁄threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

Specifications

• Accession Number: NP_005804
• For Use With (Application): Protein Assays and Analysis, Kinase Assay
• Molecular Weight (g/mol): 127.9kDa
• Quantity: 1mg
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Gene Alias: PRKCN (PKD3), PKC nu
• Assays: PRKCN was pre-diluted in enzyme dilution buffer and assayed in 50mM Tris (pH 7.5), 10mM MgCl2, 0.25mM EGTA, 0.1mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 33μM MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C
• Product Type: PRKCN (PKD3) Recombinant Human Protein
• Species: Human
• Expression System: Baculovirus
• Purity or Quality Grade: 95% by SDS-PAGE

• Concentration: 0.39mg/mL
• Formulation: Frozen
• Name: PRKCN (PKD3) Recombinant Human Protein
• Storage Requirements: –80°C
• Packaging: 1 Each
• Common Name: PRKCN (PKD3) Recombinant Human Protein
• KinaseFamily: PKN
• Specific Reactivity: 240nmoles of phosphate transferred to MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.83μg/mL
• Protein Tag: GST Tag
• Form: Frozen

For Research Use Only. Not for use in diagnostic procedures.