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Invitrogen™ Phosphotyrosine Polyclonal Antibody, HRP

Description
Phosphotyrosine Polyclonal Antibody for Western Blot, ELISA
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
Specifications
Specifications
| Antigen | Phosphotyrosine |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 1 mg/mL |
| Conjugate | HRP |
| Formulation | PBS with 4mg/mL BSA, 40% glycerol and 0.1% ProClin 300; pH 7.4 |
| Gene Alias | Phosphotyrosine; pTyr; pY |
| Host Species | Rabbit |
| Immunogen | Rabbit anti-Phosphotyrosine-HRP; HRP conjugated Polyclonal antibody specific to Multiple Phosphotyrosine [pY]. This antibody is validated for use in Western Blot, Immunoassay (ELISA). |
| Purification Method | Affinity chromatography |
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