Invitrogen™ Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

Product Code.	Quantity	unitSize
10189402	100 μL	Each

Product Code. 10189402 Supplier Invitrogen™ Supplier No. 44682G

Description

Rabbit Polyclonal Antibody

This antibody is reactive to human and rat JNK1&2. Other species of JNK1&2 have not been tested, and JNK3 (found primarily in neuronal cell lines) has not been detected. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK1&2. The final product is generated by affinity chromatography using a JNK1&2-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. Positive controls used: HEK 293 +/- UV irradiation treatment; PC12 cells +/- sorbitol.

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

Specifications

• Antigen: Phospho-JNK1/JNK2 (Thr183, Tyr185)
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene: MAPK9
• Gene Accession No.: P45983, P45984, P49185, P49186, Q91Y86, Q9WTU6
• Gene Alias: AI849689; AI851083; C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; I79_012561; JNK; JNK 55; JNK/SAPK alpha; JNK1; JNK1 beta1 protein kinase; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK-46; JNK-55; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; Mapk8; Mapk9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 9; OTTHUMP00000019552; p54 gamma; p54a; p54-alpha; p54aSAPK; Phospho-JNK; PRKM; PRKM8; Prkm9; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK; SAPK 1; SAPK gamma; SAPK1; SAPK1A; SAPK1c; SAPK-alpha; stress activated protein kinase alpha II; stress-activated protein kinase 1; stress-activated protein kinase 1a; Stress-activated protein kinase 1c; stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2
• Gene Symbols: MAPK8, MAPK9
• Host Species: Rabbit
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JNK1&2 that contains threonine 183 and tyrosine 185. This region is conserved among many species including mouse, rat, chicken, nematode, fruit fly, and in JNK3.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 116554, 26419, 26420, 50658, 5599, 5601
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG