Invitrogen™ Human PDK-1, His Tag Recombinant Protein

Product Code.	Quantity	unitSize
10104392	1 mg	1mg
15478393	100 μg	100µg

Product Code. 10104392 Supplier Invitrogen™ Supplier No. PV4033

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length.

PDK1 (pyruvate dehydrogenase kinase, isozyme 1) gene, located on chromosome 2q31.1, encodes a member of the pyruvate dehydrogenase kinase family. This kinase plays a crucial role in the regulation of glucose metabolism by phosphorylating and inactivating the pyruvate dehydrogenase complex (PDC), which converts pyruvate to acetyl-CoA, linking glycolysis to the Krebs cycle. Structurally, the PDK1 protein contains a kinase domain that is essential for its enzymatic activity. PDK1 acts as a gatekeeper of glucose oxidation and is tightly regulated by various metabolic signals, including insulin and hypoxia. Dysregulation of PDK1 activity has been implicated in metabolic disorders such as diabetes and cancer, where it contributes to altered cellular energy homeostasis and metabolic reprogramming.

Specifications

• Accession Number: Q15118
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.05% Triton X-100, 0.5mM EGTA, 20% glycerol, 100mM NaCl, 0.5mM EDTA, 5mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 5163
• Molecular Weight (g/mol): 66.9 kDa
• Name: Human PDK-1, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; B830012B01; D530020C15Rik; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDHK1; PDK p48; Pdk1; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1
• Common Name: PDK1
• Gene Symbol: PDK1
• KinaseFamily: PDHK Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Kinase Group: Ser/Thr Kinases: Atypical
• Shipping Condition: Dry Ice
• Expression System: Baculovirus
• Protein Form: Full Length
• Form: Liquid
• Research Category: Drug Development