Thermo Scientific™ MRVP Medium (Dehydrated)

Differentiate coli-aerogenes group by Methyl-red and Voges-Proskauer test with this glucose phosphate medium.

Brand:  Thermo Scientific™ CM0043B

Product Code. 10108102

Description

Differentiate coli-aerogenes group by Methyl-red and Voges-Proskauer tests with Thermo Scientific™ Oxoid™ MRVP Medium (Dehydrated). This glucose-phosphate medium is recommended for the Methyl-red and Voges-Proskauer tests, for the differentiation of the coli-aerogenes group¹. Both of these tests are based on the detection of specific breakdown products of carbohydrate metabolism.

Use MRVP Broth for differentiation of coli-aerogenes group by the Methyl-red and Voges-Proskauer test.

Smith² noted the low acid production of Enterobacter aerogenes cultures as compared with those of Escherichia coli. Clark & Lubs³ employed methyl-red as a hydrogen-ion concentration indicator in order to differentiate glucose phosphate peptone water cultures of members of the coli-typhoid group. This test, now known as the Methyl-red test, distinguishes those organisms able to form large amounts of acid from glucose so that the pH falls below 4.4 and those organisms which cannot produce a low pH level.

The difference in pH value is visualised by adding methyl-red to the culture, (<pH 4.4 red: pH 5.0-5.8 orange: >pH 6.0 yellow).

Voges & Proskauer⁴ described a red fluorescent coloration that appeared after the addition of potassium hydroxide to cultures of certain organisms in glucose medium. The coloration was shown to be due to the oxidation of the acetylmethyl-carbinol producing diacetyl which reacts with the peptone of the medium to give a red colour^5,6. Durham⁷ noted that Enterobacter aerogenes gave a positive reaction but that Escherichia coli produced no coloration, and it later became clear that there was a negative correlation between the Methyl-red and Voges-Proskauer tests^8,9 for lactose-fermenting coliform organisms.

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Specifications

• Description: MRVP Medium (Clarks and Lubs Medium)
• Product Type: Dehydrated Culture Media
• Yield: For 29.4L medium

• Form: Powder
• Quantity: 500 g

For Laboratory Use Only