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Macherey-Nagel™ NucleoBond™ RNA/DNA 400, Maxi kit with anion exchange technology

Product Code. 10181291
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Product Code. 10181291 Supplier Macherey-Nagel™ Supplier No. 740651

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Description

Anion exchange chromatography for nucleic acids of highest integrity.

NucleoBond™ RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80μg or 400μg ultra pure total RNA. A special manufacturing process guarantees RNase-free handling. Due to the superior resolving power of NucleoBond™ anion exchanger contaminations are washed away, and RNA and genomic DNA can be eluted separately.

Specific references:

M. Jiang et al. , J. Virol. Methods

38

(1992) 205T. Roitsch et al. , Plant Physiol.

108

(1995) 285D. Godt et al. , J. Plant Physiol.

146

(1995) 231R. Ehness et al. , The Plant Cell

9

(1997) 1825S. P. Hale et al. , Science

276

(1997) 1250Kit components: NucleoBond™ RNA/DNA 80 - NucleoBond™ AXR 80 columns, all required buffers. NucleoBond™ RNA/DNA 400 - NucleoBond™ AXR 400 columns, all required buffers.

  • Anion-exchange technology
  • Typical RNA yield: 70μg to 300μg from cultured cells 30μg to 150μg from tissue 50μg to 200μg from bacteria
  • Sample material: eukaryotic cultured cells, tissue, bacteria/yeast cells
  • Fragment size: 50bp to 300kb
  • Binding capacity: 80μg for NucleoBond RNA/DNA 80 400μg for NucleoBond RNA/DNA 400
  • Preparation time: 1.5 hr. to 2. 5 hr.
  • Format: gravity-flow columns - midi or maxi

Specifications

No. of Reactions 10 Preps
Product Type RNA/DNA 400

Safety and Handling

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Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H412-Harmful to aquatic life with long lasting effects.
 Supplemental information
  • MIXTURE LIST-Contain: ethanol, guanidiniumthiocyanate, tritonx-100
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