missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Extraction and Purification
  5. Macherey-Nagel™ NucleoBond™ CB 20/CB 100, Midi kit for high integrity DNA from cells and blood

Macherey-Nagel™ NucleoBond™ CB 20/CB 100, Midi kit for high integrity DNA from cells and blood

Product Code. 10121251
Change view
Click to view available options
Yield:
100 μg
5-20 μg
Column Type:
NucleoBond AXG 100
NucleoBond AXG 20
Unit Size:
20 reactions
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Yield Column Type unitSize
10121251 100 μg NucleoBond AXG 100 20 reactions
11922452 5-20 μg NucleoBond AXG 20 20 reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 10121251 Supplier Macherey-Nagel™ Supplier No. 740508

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Anion exchange chromatography for purification of DNA from whole blood and cultured cells.

Genomic DNA quality is extraordinarily high. Samples are first lysed under special conditions for removal of erythrocytes. After incubation with proteinase K, the sample is diluted with buffer N2 before loading onto the NucleoBond™ column. After elution with high-salt buffer the genomic DNA is precipitated with isopropanol. The DNA purified by this method is suitable for all kinds of subsequent reactions. Kit components: NucleoBond™ CB 100 kit - NucleoBond AXG 100 columns, buffers, proteinase K. NucleoBond™ CB 20 kit - NucleoBond™ AXG 20 columns, buffers, proteinase K.
  • Anion exchange technology
  • Format: gravity flow columns
  • Sample material: whole blood, buffy coat, cultured cells (up to 20 mL blood)
  • Fragment size: 500bp to 300kb
  • Yield: 100μg or 5-20μg depending on column used
  • Binding capacity: 100μg or 5-20μg depending on column used
  • Preparation time: 4 to 5hrs For purification of up to 500μg genomic DNA from whole blood.

Specifications

Column Type NucleoBond AXG 100
Format Mini Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
For Use With (Application) Applications: Genomic DNA from whole blood, serum, plasma, cultured cells, buffy coat.
No. of Reactions 20 preps
Product Type NucleoBond™ CB
Size 500kbp
Test Time 4-5hr.
Yield 100 μg

Safety and Handling

compliance-icons compliance-icons compliance-icons
Hazard Category
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.