Macherey-Nagel™ NucleoBond™ CB 500, Midi kit for high integrity DNA from cells and blood

Product Code. 10036714 Supplier Macherey-Nagel™ Supplier No. 740509

Description

Anion exchange chromatography for purification of DNA from whole blood and cultured cells.

Genomic DNA quality is extraordinarily high. Samples are first lysed under special conditions for removal of erythrocytes. After incubation with proteinase K, the sample is diluted with buffer N2 before loading onto the NucleoBond™ column. After elution with high-salt buffer the genomic DNA is precipitated with isopropanol. The DNA purified by this method is suitable for all kinds of subsequent reactions.Kit components: NucleoBond™ CB 100 kit - NucleoBond AXG 100 columns, buffers, proteinase K. NucleoBond™ CB 500 kit - NucleoBond™ AXG 500 columns, buffers, proteinase K.

• Anion exchange technology
• Format: gravity flow columns
• Sample material: whole blood, buffy coat, cultured cells (up to 20 mL blood)
• Fragment size: 500bp to 300kb
• Yield: 100μg or 500μg depending on column used
• Binding capacity: 100μg or 500μg depending on column used
• Preparation time: 4 to 5hrs

For purification of up to 500μg genomic DNA from whole blood.

Specifications

• Format: Midi Gravity-flow Column
• Isolation Technology: Anion Exchange Chromatography Technology
• For Use With (Application): For High-copy Plasmids From E. coli
• No. of Reactions: 10
• Product Type: NucleoBond™ AXG 500 Columns
• Test Time: 4 to 5hr.
• Yield: 500μg

Safety and Handling

Hazard Category

• Respiratory sensitiser Category 1
• Skin sensitiser Category 1