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Invitrogen™ PRAS40 (Phospho) [pT246] Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHO0421

366.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.31000kg

Product Code. 10069112

  • £550.00 / 96 tests
Estimated Shipment: 23-04-2024
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For Research Use Only. All usage must comply with product instructions.
 

Includes: PRAS40 Antibody Coated 96-Well Plate, PRAS40 [pT246] Standard, Standard Diluent Buffer, PRAS40 [pT246] Detection Antibody, Anti-Rabbit IgG-HRP (100X), HRP Diluent, Wash Buffer Concentrate (25X), Stabilized Chromogen, TMB, Stop Solution, Plate Covers, Detailed protocol with validation tests

Description

Description

The Human PRAS40 (Phospho) [pT246] ELISA Kit is designed to detect and quantify the level of PRAS40 (Phospho) [pT246] in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human PRAS40 (Phospho) [pT246]. Principle of the method A monoclonal capture antibody specific for PRAS40 (Phospho) [pT246] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.

Order Info

Shipping condition: Wet ice

Specifications

Specifications

Q96B36
<0.5 U/mL
HRP
Cell Lysates
Human
84335
4 hr.
4.7%
HRP
96 Tests
Cell Lysate,10 μL
Human
4 hr.
1.6-100 U/mL
1.6U/mL
ELISA Kit
Adaptor Proteins
Colorimetric Microplate Reader
Lobe,PRAS40
5.9%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
PRAS40
RUO
2°C to 8°C
1 hr. 20 min.
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For research use only. Not intended for human or animal therapeutic or diagnostic use