Invitrogen™ Rat IL-12 p70 ELISA Kit

Product Code.	Quantity	unitSize
10190913	96 Tests	Each

Product Code. 10190913 Supplier Invitrogen™ Supplier No. KRC2371

Description

ELISA

The Rat Interleukin-12 p70 (Rt IL-12 p70) ELISA quantitates IL-12 in rat serum, EDTA plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-12 heterodimer. Principle of the method The Rat IL-12 p70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

Specifications

• Accession Number: Q9R103
• Assay Range: 7.8 to 500 pg/mL
• Assay Sensitivity: <2.5 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Rat
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 64546, 84405
• Gene Symbol: Il12a, Il12b
• Interassay CV: 4%
• Intraassay CV: 3.3
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Precision: <2.5 pg/mL
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-12 p70
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs