Invitrogen™ Human IL-12 p70 ELISA Kit

ELISA

Brand:  Invitrogen™ KAC1568

Product Code. 10100863

Description

The Human Interleukin-12 (Hu IL-12 p70) ELISA quantitates Hu IL-12 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL–12 heterodimer.

Principle of the method The Human IL-12 p70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

Specifications

• Accession Number: P29459,P29460
• Assay Sensitivity: 0.2 pg/mL
• Product Type: ELISA
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CLMF,CLMF2,IL-12 B,IMD28,IMD29,NKSF,NKSF2,CLMF,IL-12 A,NFSK,NKSF1,P35
• Intraassay CV: 7.3
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Target: IL-12 p70
• Total Assay Time: 4 hrs

• Assay Range: 1.56 to 100 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric
• Gene ID (Entrez): 3592, 3593
• Interassay CV: 7.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
• Precision: 0.5 pg/mL
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins