Gibco™ Mouse IL-15 Recombinant Protein

Product Code.	Quantity	unitSize
10170873	10 μg	Each

Product Code. 10170873 Supplier Gibco™ Supplier No. PMC0155

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute mouse IL-15 in sterile, distilled water to a concentration of 0.5-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 6 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

Specifications

• Accession Number: P48346
• For Use With (Application): Bioactivity
• Formulation: 10mM sodium phosphate with no preservative; pH 8
• Gene ID (Entrez): 16168
• Molecular Weight (g/mol): 13.4 kDa
• Name: Mouse IL-15
• pH Range: 8
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: AI503618; il 15; Il15; IL-15; ILN; Interleukin; interleukin 15; Interleukin15; interleukin-15; MGC9721; M-IL-15
• Common Name: IL-15
• Gene Symbol: IL15
• Biological Activity: ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Sequence: Mouse IL-15 recombinant protein contains 115 amino acids
• Content And Storage: -20°C
• Activity: ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Cell Cycle & Proliferation, Oncology, Immunology, Signal Transduction
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC