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Invitrogen™ T4 DNA Ligase (1 U/µL)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Brand:  Invitrogen™ 15224017

44.62 GBP valid until 2021-10-30
Use promo code "14806" to get your promotional price.



Code : 50

Additional Details : Weight : 0.00010kg

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Product Code. 10124752

  • £46.97 / Each

In stock, ready to ship: 14

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.

  • Optimizes ligation in 5 minutes
  • Source: Purified from E. coli lambda lysogen NM989
  • Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
  • Unit definition: One unit catalyzes exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
  • Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2, 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning

Specifications

Specifications

100 U
1 U/μL
Ligase
T4 DNA Ligase
Dry Ice
DNA Ligase Buffer, 5X Reaction Buffer
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For Research Use Only. Not for use in diagnostic procedures.