Gibco™ Human FGF-19 Recombinant Protein

Product Code.	Quantity	unitSize
10110683	25 μg	Each

Product Code. 10110683 Supplier Gibco™ Supplier No. PHG0244

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity. The orthologous protein in mouse is FGF15, which shares about 50% amino acid identity and has similar functions. Together they are often referred to as FGF15/19.

Specifications

• Accession Number: O95750
• For Use With (Application): Bioactivity
• Formulation: 5mM tris with 75mM NaCl and no preservative; pH 7.6
• Gene ID (Entrez): 9965
• Molecular Weight (g/mol): 21.8 kDa
• Name: Human FGF-19
• pH Range: 7.6
• Purification Method: Purified
• Quantity: 25 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: FGF; Fgf15; FGF19; FGF-19; Fibroblast growth factor; fibroblast growth factor 15; fibroblast growth factor 19; UNQ334/PRO533
• Common Name: FGF19
• Gene Symbol: FGF19
• Biological Activity: ED50 = 100 - 150 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human FGF-19 recombinant protein contains 195 amino acids
• Content And Storage: -20°C
• Activity: ED50 = 100 - 150 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: FGFs (Fibroblast Growth Factors)
• Research Category: Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis
• Product Line: Gibco
• Purity or Quality Grade: ≥95% by SDS-PAGE and HPLC