Gibco™ Human Cardiotrophin-1 Recombinant Protein

Product Code. 10140633

Brand: Gibco™ PHC1594

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute Cardiotrophin-1 in 20 mM Tris, pH 8.0, to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA. Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.

Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development.

Specifications

• Accession Number: Q16619
• For Use With (Application): Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 1489
• Molecular Weight (g/mol): 21.5 kDa
• Name: Human Cardiotrophin-1
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: cardiophin 1; Cardiotrophin; cardiotrophin 1; Cardiotrophin1; cardiotrophin-1; CT 1; CT1; CT-1; CT-1CT1; CTF 1; Ctf1; IL-6 cytokine family
• Common Name: Cardiotrophin 1
• Gene Symbol: CTF1
• Biological Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human Cardiotrophin-1 recombinant protein contains 200 amino acids
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Neurobiology, Stem Cell Research, Cardiovascular Research, Angiogenesis
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC