Gibco™ Human Active BMP-7 Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL (Do not vortex) and store at 2-8°C for up to 1 week. Make any further dilutions of the reconstituted protein in buffer containing carrier protein, such as 0.1% BSA, and apportion into working aliquots to store at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month. Store the lyophilized protein at -20°C to -80°C,preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. Working aliquots stored with a carrier protein are stable for at least 7 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis, BMP7 has a proposed role in early development. In addition, the fact that BMP7 is closely related to BMP5 and BMP6 has lead to speculation of possible bone inductive activity.

Specifications

• Accession Number: P18075
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 655
• Molecular Weight (g/mol): 28.8 kDa
• Name: Human Active BMP-7
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: BMP; BMP7; BMP-7; bone moorphogenic protein-7; Bone morphogenetic protein; bone morphogenetic protein 7; bone morphogenetic protein 7 (osteogenic protein 1); bone morphogenetic protein 7 preproprotein; bone morphogenic protein 7; Eptotermin alfa; OP1; OP-1; Osteogenic protein 1; RP11-560A15.5
• Common Name: BMP-7
• Biological Activity: ED50 = 0.02 - 0.04 ng/mL; determined by the dose-dependent induction of alkaline phosphatase in mouse ATDC52 chondrogenic cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human BMP-7 recombinant protein is a homodimer containing two 117 amino acid subunits (BMP-7, amino acids 315-431)
• Content And Storage: -20°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: CHO cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: BMPs (Bone Morphogenetic Proteins) & Related Factors
• Research Category: Bone Research, Stem Cell Research, Differentiation
• Product Line: Gibco™
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC