Gibco™ Ham's F-10 Nutrient Mix, HEPES
Ham's F-10 Nutrient Mix, HEPES
Brand: Gibco™ 22390025
Code : 50
Additional Details : Weight : 0.60200kg
DescriptionHam's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ F-10 product made in our Grand Island facility (12390-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10%Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
|Mammalian cell culture|
|With-L-glutamine, Phenol Red, HEPES|
|Ham's F-10 Nutrient Mix|
|2 to 8°C|
|L-glutamine, HEPES, phenol red, sodium pyruvate|
|7.0 to 7.4|
|Ham's F-10 Nutrient Mix, 500mL, 1X Concentration, Liquid, 7.0 to 7.4 pH, 12 Months Shelf Life, 270 to 320 mOsm/kg Osmolality, With Sodium Pyruvate, L-glutamine, Phenol Red, HEPES, 2 to 8°C Storage, For Mammalian Cell Culture|
|2 to 8°C|
|ISO 13485 standards, FDA|