Fluorescein-MAP2K1

Fluorescein-MAP2K1

Brand:  Invitrogen PV4812

Product Code. 10144603

Description

Inactive MAP2K1 (MEK1) has been labeled with fluorescein, and is a substrate for MAPK family members.

For use in combination with the LanthaSreen™ Tb-MAP2K1 [pSer217/221] Antibody (PV4813) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

• Assay Entry: Biochemical terbium phospho-specific activity
• Detection Method: Fluorescent
• Substrate: MAP2K1
• Substrate Type: Kinase Substrate
• Gene ID (Entrez): 10454, 10746, 1326, 4215, 4217, 4293, 4294, 4296, 5894, 673, 6885
• Molecular Weight (g/mol): 50000 Da
• Target Entry: MAP3K2, RAF1 (cRAF) Y340D Y341D, MAP3K10, MAP3K9, MAP3K7/MAP3K7IP1, MAP3K5, MAP3K11, BRAF V599E, BRAF, MAP3K8, MAP3K3

• Content And Storage: Fluorescein-MAP2K1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1mM DTT. Store at -80°C.
• Quantity: 20 nmol
• Substrate Properties: Protein-Based Substrate
• Target Enzyme: Serine/Threonine Kinases
• Label or Dye: FITC (Fluorescein)
• Shipping Condition: Dry Ice
• Validated Kinases: BRAF, MAP3K2, MAP3K3, MAP3K5, MAP3K7, MAP3K8, MAP3K9, MAP3K10, MAP3K11

For Research Use Only. Not for use in diagnostic procedures.