Invitrogen™ FL - SRC 1-4 Peptide

Product Code.	Quantity	unitSize
10056532	100 μL	Each

Product Code. 10056532 Supplier Invitrogen™ Supplier No. PV4582

Description

Contains known interaction motifs and labeled with fluorescein. Invitrogen™ Fluorescein-SRC 1-4 Peptide, 100μM is matched to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays to enable primary or secondary screening of nuclear receptor agonists and antagonists.

Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide.

In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide. The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio.

Specifications

• Content And Storage: Store in freezer (-5 to -30°C).
• Label Type: Classic Dyes
• Quantity: 100 μL
• Shipping Condition: Dry Ice

For Research Use Only. Not for use in diagnostic procedures.