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Gibco™ DMEM, high glucose

DMEM, high glucose

Brand:  Gibco™ 41965047

Code : 50

Additional Details : Weight : 1.00000kg

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Product Code. 10063542

  • £24.20 / 1 L

EU Stock 386

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM product made in our Grand Island facility (11965-084). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications

Specifications

ISO 13485 standard
Dulbecco's Modified Eagle Medium (DMEM), 1000mL, Liquid, 1X Concentration, 7.0 to 7.4 pH, With High Glucose, L-Glutamine, Phenol Red, 12 Months Shelf Life, 320 to 360mOsm/kg Osmolality, For Mammalian Cell Culture
Liquid
With - High glucose, L-glutamine, phenol red, without - Sodium pyruvate, HEPES
Dulbecco's Modified Eagle Medium
2 to 8°C
HEPES, sodium pyruvate
1 x
Mammalian cell culture
Liquid
7.0 to 7.4
1000mL
High glucose, L-glutamine, phenol red
2 to 8°C
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